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Primary Antibodies  >  Cancer Research Antibodies
 

CD20 Allophycocyanin Antibody

Mouse Monoclonal 2H7 IgG2b kappa
Code

200-326-N64
Size

500 µL
Price

$225.00
Availability

Ships within 2 weeks.
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E-mail: orders@rockland-inc.com
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Flow Cytometry of Mouse anti-CD20 Allophycocyanin Conjugated Monoclonal Antibody.
Cells:  human peripheral blood lymphocytes.  
Stimulation:  none. 
Antibody:  (Dotted Line) APC Mouse IgG2b kappa isotype control; (Solid Line) Allophycocyanin Anti-CD20 mouse antibody using 5 ul.
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Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20

CD20 Allophycocyanin Antibody Properties

Description
Anti-CD20 (MOUSE) Allophycocyanin Conjugated Monoclonal Antibody - 200-326-N64
Host
Mouse
Known Cross Reactivity
Human
Clonality
Monoclonal 2H7 IgG2b kappa
Application
FlowCytometry : 5 ul/1x10e6 cells or 100µL of whole blood
Immunochemistry: User Optimized
ImmunoPrecipitation: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG2b
FP Value
1-2 moles Allophycocyanine per mole of IgG2b
Concentration
Sufficient to run approximately 100 tests
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.09% (w/v) Sodium Azide
Stabilizer
0.2% BSA (w/v)
GeneName
MS4A1
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Lot#  

CD20 Allophycocyanin Antibody Description

Background
CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.
Immunogen
Anti-CD20 Antibody (Monoclonal) was produced by repeated immunizations with CD20 antigen.
Storage Condition
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
Application Note
Anti-CD20 is useful for Flow Cytometry, Immunoprecipitation, and Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.
Purity/Specifity
Allophycocyanin conjugated CD20 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD20. Reactivity is observed against human CD20 Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, and Squirrel Monkey. Cross reactivity with CD20 from other sources has not been tested. Anti-CD20 is conjugated with APC under optimal conditions and the solution is free of unconjugated APC.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Specific Reference
Hultin L, et al. 1993. Cytometry 14:196. Tedder T, et al. 1994. Immunol. Today 15:450.
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Product Type
Proteins and Peptides;
Applications
ELISA, FLISA, WB, IF, FC
Catalog Number
010-001-332

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human, chimpanzee
Catalog Number
200-401-908

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-1302

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
human
Catalog Number
200-301-J34

Product Label

 

Description
Allophycocyanine
Color
Far-Red
Conjugation Reference
Modified from the method of Carlsson J, Drevin H, and Axen R. Protein thiolation and reversible protein-protein conjugation. Biochem J 173, 723-737 (1978).
Molecular Weight
100000
Excitation Wavelength
650
Conjugation Chemistry
SATA/MBS