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Primary Antibodies  >  Phycoerythrin Conjugated Antibodies
 

CD8a Phycoerythrin Antibody

Mouse Monoclonal HIT8a IgG1 kappa
Code

200-308-N61
Size

500 µL
Price

$279.00
Availability

Ships within 2 weeks.
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E-mail: orders@rockland-inc.com
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Flow Cytometry of Mouse anti-CD8a Phycoerythrin Conjugated Monoclonal Antibody.
Cells:  human peripheral blood lymphocytes.  
Stimulation:  none. 
Antibody:  (PURPLE) PE Mouse IgG1 isotype control; (GREEN) Phycoerythrin Anti-CD8a mouse antibody using 5 ul.
Shipping & Handling Fees Apply.

Shipping:
$20.00 to United States
Handling:
$20.00
Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a

CD8a Phycoerythrin Antibody Properties

Description
Anti-CD8a (MOUSE) Phycoerythrin Conjugated Monoclonal Antibody - 200-308-N61
Host
Mouse
Known Cross Reactivity
Human
Clonality
Monoclonal HIT8a IgG1 kappa
Application
Flow Cytometry : 5 ul/1x10e6 cells or 100µL of whole blood
Immunohistochemistry: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG1
FP Value
1-2 moles R-Phycoerythrin (RPE) per mole of IgG1
Concentration
Sufficient to run approximately 100 tests
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.09% (w/v) Sodium Azide
Stabilizer
0.2% BSA (w/v)
GeneName
CD8A
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Lot#  

CD8a Phycoerythrin Antibody Description

Background
CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the a3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
Immunogen
Anti-CD8a Antibody (Monoclonal) was produced by repeated immunizations with CD8a antigen.
Storage Condition
Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. DO NOT FREEZE. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Application Note
Anti-CD8a is useful for Immunohistochemistry and Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.
Purity/Specifity
Phycoerythrin conjugated CD8a Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD8a. Reactivity is observed against human CD8a, Marmoset, and chimpanzee. This clone recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a. Cross reactivity with CD8a from other sources has not been tested. Anti-CD8a is conjugated with PE under optimal conditions and the solution is free of unconjugated PE.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego. Awasthi, S., et al. 2011. J. Virol 85:10472. PubMed Coppieters KT, et al. 2012. J. Exp. Med. 209:51. Suzuki F, et al. 2012. Arthritis Res. Ther. 14:R48.
Specific Reference
Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
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Product Type
Proteins and Peptides;
Applications
ELISA, FLISA, WB, IF, FC
Catalog Number
010-001-332

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human, chimpanzee
Catalog Number
200-401-908

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-1302

Product Type
Secondary Antibodies;
Applications
FLISA, WB, IHC, IF, FC
Reacts With
Mouse
Catalog Number
610-102-041

Product Label

 

Description
R-Phycoerythrin (RPE)
Color
Red
Conjugation Reference
Modified from the method of Carlsson J, Drevin H, and Axen R. Protein thiolation and reversible protein-protein conjugation. Biochem J 173, 723-737 (1978).
Molecular Weight
240000
Excitation Wavelength
488
Conjugation Chemistry
SATA/MBS