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Primary Antibodies  >  Cancer Research Antibodies
 

CD47 Fluorescein Antibody

Mouse Monoclonal B6H12.2 IgG1
Code

200-302-M68
Size

250 µL
Price

$165.00
Availability

Ships within 2 weeks.
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Cell Surface Flow Cytometry of Mouse anti-HUMAN CD47 antibody Fluorescein conjugated.  
Cells: 5 x 10^5 human PBMC. 
Stimulation: none.
Antibody:  (GREEN) isotype control antibody;  (RED) Fluorescein Anti-CD47 mouse secondary antibody using 10µL.
Shipping & Handling Fees Apply.

Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Protein MER6, CD47

CD47 Fluorescein Antibody Properties

Description
Anti-CD47 (MOUSE) Fluorescein Conjugated Monoclonal Antibody - 200-302-M68
Host
Mouse
Target Species
Human
Known Cross Reactivity
Human
Clonality
Monoclonal B6H12.2 IgG1
Application
FlowCytometry : 10 µL/5 x 10^5 cells (0.5 µg)
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG1
FP Value
2-8 moles Fluorescein (FITC) per mole of IgG1
Concentration
Sufficient to run approximately 25 tests
Buffer
0.01 M Tris Cl, 0.15 M Sodium Chloride, pH 8.0
Preservative
0.05% (w/v) Sodium Azide
Stabilizer
0.05% BSA
GeneName
CD47
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Lot#  

CD47 Fluorescein Antibody Description

Background
CD47, originally named integrin-associated protein (IAP), is a 50-kDa protein containing five membrane spanning sequences and a short cytoplasmic tail. CD47 appears to be important in myeloid cell activation and migration across endothelial and epithelial monolayers. Anti-CD47 antibody has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47 and its ligands, thrombospondin 1 (TSP-1) and SIRP-a, are important regulators of dendritic cells. Recently, it has been shown that CD47 and TLR2 cross-talk to regulate PMN transmigration. The activation of TLR2/6 by MALP-2, a TLR2 specific agonist, potently inhibits human and murine PMN transmigration. The cross-talk between TLR2 and CD47 may be mediated through MYD88.
Immunogen
Anti-CD47 Antibody (Monoclonal) was produced by repeated immunizations with CD47 antigen.
Storage Condition
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
Application Note
Anti-CD47 is useful for Flow Cytometry (Cell Surface). Researchers should determine optimal titers for applications that are not stated.
Purity/Specifity
Fluorescein conjugated CD47 Monoclonal Antibody was Protein G Purified and is directed against human CD47. Reactivity is observed against human CD47. Cross reactivity with CD47 from other sources has not been tested.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
1. Sarfati M, Fortin G, Raymond M, Susin S. CD47 in the immune response: role of thrombospondin and SIRP-alpha reverse signaling. Curr Drug Targets, 9(10): 842-850 (2008). 2. Chin AC, Fournier B, Peatman EJ, Reaves TA, Lee WY, Parkos CA. CD47 and TLR-2 cross-talk regulates neutrophil transmigration. J Immunol., 183(9): 5957-5963 (2009). 3. Griffiths M, Neal JW, Gasque P. Innate immunity and protective neuroinflammation: new emphasis on the role of neuroimmune regulatory proteins. Int Rev Neurobiol. 82:29-55 (2007). 4. Joanna C. Porter and Nancy Hogg. Integrins take partners: cross-talk between integrins and other membrane receptors. Trends in Cell Biology 8 (10): 390-396 (1998). 5. D Cooper, FP Lindberg, JR Gamble, EJ Brown, and MA Vadas.  Transendothelial migration of neutrophils involves integrinassociated protein (CD47). Proc. Natl. Acad. Sci. USA,  92: 3978-3982 (1995).
Specific Reference
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Product Type
Proteins and Peptides;
Applications
ELISA, FLISA, WB, IF, FC
Catalog Number
010-0140

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human, chimpanzee
Catalog Number
200-401-908

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-10

Product Type
Molecular Biology Reagents;
Catalog Number
MB-008

Product Label

 

Description
Fluorescein (FITC)
Color
Green
Conjugation Reference
Modified from The and Feltkamp, Immunology 18; 865, 1970.
Molecular Weight
390
Excitation Wavelength
495
Conjugation Chemistry
Isothiocyanate