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Primary Antibodies  >  Cancer Research Antibodies
 

CD8 APC Antibody

Mouse Monoclonal RPA-T8 IgG1 kappa
Code

200-326-M64
Size

500 µL
Price

$199.00
Availability

Ships within 2 weeks.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Cell Surface Flow Cytometry of Mouse anti-HUMAN CD8 antibody Allophycocyanin conjugated.  
Cells:  10^6 human PBMC. Propidium iodide negative lymphocyte population gated for analysis.
Stimulation: none.
Antibody:  (GREEN) isotype control antibody; (RED) Allophycocyanin Anti-CD8 mouse secondary antibody using 10 ul (0.1 ug).
Shipping & Handling Fees Apply.

Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a, CD8A, MAL

CD8 APC Antibody Properties

Description
Anti-CD8 (MOUSE) Allophycocyanin Conjugated Monoclonal Antibody - 200-326-M64
Host
Mouse
Target Species
Human
Known Cross Reactivity
Human
Clonality
Monoclonal RPA-T8 IgG1 kappa
Application
FlowCytometry : 10 µL/10^6 cells (0.25 µg)
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG1
FP Value
1-2 moles Allophycocyanine per mole of IgG1
Concentration
0.05 mg/mL Sufficient to run approximately 100 tests
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.09% (w/v) Sodium Azide
Stabilizer
0.1% Gelatin
GeneName
CD8
Download CofA
Lot#  

CD8 APC Antibody Description

Background
CD8 is a cell surface glycoprotein expressing on thymocytes subsets and cytotoxic T cells and is either a homodimer of two alpha chains, or a heterodimer of one alpha and one beta chain. It acts as a coreceptor during T cell activation through the binding of MHC Class I molecules and is found on T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 associates with LCK with the help of a zinc clasp structure and down-regulates the production of major Th2-type cytokines. CD8 is also known to recruit pUL97 thereby inducing dissolution of the nuclear lamina and facilitating the nuclear export of viral capsids.
Immunogen
Anti-CD8 Antibody (Monoclonal) was produced by repeated immunizations with CD8 antigen.
Storage Condition
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
Application Note
Anti-CD8 is useful for Flow Cytometry (Cell Surface). Researchers should determine optimal titers for applications that are not stated.
Purity/Specifity
Allophycocyanin conjugated CD8 Monoclonal Antibody was Protein G Purified from tissue culture supernatant and is directed against human CD8. Reactivity is observed against human CD8, chimpanzee, baboon, cynomolgus, rhesus, and ooty mangabey. Cross reactivity with CD8 from other sources has not been tested.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
1. Marschall, M. et al. J. Biol. Chem. 280:33357-33367 (2005). 2. Kim, PW. et al. Science. 301:1725-1728 (2003). Estes JD, Gordon SN, Zeng M, Chahroudi AM, Dunham RM, Staprans SI, Reilly CS, Silvestri G, and Haase AT. 2008. J. Immunol. 180: 6798-6807. Chlereth B, Fichtner I, Lorenczewski G, Kleindienst P, Brischwein K, da Silva A, Kufer P, Lutterbuese R, Junghahn I, Kasimir-Bauer S, Wimberger P, Kimmig R and Baeuerle PA. 2005. Cancer Res. 65: 2882-2889. Mack CL, Tucker RM, Sokol RJ, Darrer FM, Kotzin BL, Whitington PF and Miller SD. 2004. Pediatr. Res. 56(1):79-87. Huang Z-Y, Hunter S, Kim M-K, Chien P, Worth RG, Indik ZK, and Schreiber AD. 2004. J. Leukoc. Biol. 76:491-499 Kayagaki N, Yamaguchi N, Nagao F, Matsuo S, Maeda H, Okumura K, and Yagita H. 1997. Proc. Natl. Acad. Sci. 94:3914-3919. Deng MC, Bell S, Huie P, Pinto F, Hunt SA, Stinson EB, Sibley R, Hall BM, and Valantine HA. 1995. Circulation. 91: 1647-1654.
Specific Reference
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Product Type
Proteins and Peptides;
Applications
ELISA, FLISA, WB, IF, FC
Catalog Number
010-001-330

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human, chimpanzee
Catalog Number
200-401-908

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-10

Product Type
Molecular Biology Reagents;
Catalog Number
MB-008

Product Label

 

Description
Allophycocyanine
Color
Far-Red
Conjugation Reference
Modified from the method of Carlsson J, Drevin H, and Axen R. Protein thiolation and reversible protein-protein conjugation. Biochem J 173, 723-737 (1978).
Molecular Weight
100000
Excitation Wavelength
650
Conjugation Chemistry
SATA/MBS