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Primary Antibodies  >  Cancer Research Antibodies
 

CD3 APC Antibody

Mouse Monoclonal OKT3 IgG2a kappa
Code

200-326-M62
Size

500 µL
Price

$215.00
Availability

Ships within 2 weeks.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Flow Cytometry of Mouse anti-HUMAN CD3 antibody Allophycocyanin conjugated.  
Cells:  10^6 human PBMC.  (Shaded area cells without antibody).
Stimulation: none.
Antibody:  (GREEN) isotype control antibody; (RED) Allophycocyanin Anti-CD3 mouse secondary antibody using 10 ul.
Shipping & Handling Fees Apply.

Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e

CD3 APC Antibody Properties

Description
Anti-CD3 (MOUSE) Allophycocyanin Conjugated Monoclonal Antibody - 200-326-M62
Host
Mouse
Target Species
Human
Known Cross Reactivity
human
Clonality
Monoclonal OKT3 IgG2a kappa
Application
FlowCytometry : 10 µL/1x10^6 cells
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG2a
FP Value
1-2 moles Allophycocyanine per mole of IgG2a
Concentration
0.025 mg/mL Sufficient to run approximately 100 tests
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.09% (w/v) Sodium Azide
Stabilizer
0.1% Gelatin
GeneName
CD3e
Download CofA
Lot#  

CD3 APC Antibody Description

Background
CD3 (T3), a complex T cell marker, is known to associate noncovalently with the a/b or g/z heterodimer of the T cell antigen receptor (TCR) to form the most complex transmembrane (TM) receptor structures. Structurally, CD3 consists of four distinct membrane glycoprotein isoforms (CD3g, CD3e, CD3s and the zeta chain) to generate activation signals. CD3 is specially engaged in antigen recognition and is known to play an important role in mediating signals that are critical for T cell development in the thymus, proliferation, and induction of T cell-mediated immune responses against infectious agents and also in the differentiation of T cells into effector and memory populations. CD3 usually expresses in the cytoplasm of prothymocytes, and on the surface of about 95% of thymocytes, but cytoplasmic CD3 is lost as the cells differentiate into medullary thymocytes. Apart from its role as an important marker in the classification of malignant lymphomas and lymphoid leukaemia, CD3 can also be useful for the identification of T cells in celiac disease, lymphocytic colitis and colorectal carcinomas associated with loss of a mismatch repair protein. CD3 indirectly plays an important role in immunomodulation whereas the anti-CD3 antibody may be used in in vitro Treg assays to generate effector T cells.
Immunogen
Anti-CD3 Antibody (Monoclonal) was produced by repeated immunizations with CD3 antigen.
Storage Condition
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
Application Note
Anti-CD3 is useful for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.
Purity/Specifity
Allophycocyanin conjugated CD3 Monoclonal Antibody was Protein G Purified and is directed against human CD3. Reactivity is observed against human CD3 and chimpanzee. Cross reactivity with CD3 from other sources has not been tested.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
1. Evans G. A. et al. Immunogenetics 28: 365-373, (1988). 2. Brygida Bisikirska et al. J Clin Invest. 115: 2904–2913, (2005). Haga-Friedman A, Horovitz-Fried M, and Cohen CJ. Jun 2012. J. Immunol. 188:5538-5546. Bikker A, Moret FM, Kruize AA, Bijlsma JWJ, Lafeber FPJG, and van Roon JAG. Jun 2012. Rheumatology 51:996-1005 Bagnara D, Kaufman MS, Calissano C, et al. 2011. Blood. 117: 5463-5472. Nguyen V, Cao L, Lin JT, Hung N, Ritz A, Yu K, Jianu R, Ulin SP, Raphael BJ, Laidlaw DH, Brossay L, and Salomon AR. 2009. Mol. Cell. Proeomics. 8: 2418-2431. Bibollet-Ruche F, McKinney BA, Duverger A, Wagner FH, Ansari AA, and Kutsch O. 2008. J. Virol. 82(20): 10271-10278. Roura-Mir C, Catalfamo M, Cheng TY, Marqusee E, Besra GS, Jaraquemada D, and Moody DB. 2005. J. Immunol. 174:3773-3780 Sato Y, Mukai K, Watanabe S, Goto M, and Shimosato Y. 1986. Am. J. Pathol.
Specific Reference
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Product Type
Proteins and Peptides;
Applications
ELISA, FLISA, WB, IF, FC
Catalog Number
010-001-332

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human, chimpanzee
Catalog Number
200-401-908

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-10

Product Type
Molecular Biology Reagents;
Catalog Number
MB-008

Product Label

 

Description
Allophycocyanine
Color
Far-Red
Conjugation Reference
Modified from the method of Carlsson J, Drevin H, and Axen R. Protein thiolation and reversible protein-protein conjugation. Biochem J 173, 723-737 (1978).
Molecular Weight
100000
Excitation Wavelength
650
Conjugation Chemistry
SATA/MBS