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Primary Antibodies  >  Cancer Research Antibodies
 

CD4 Fluorescein Antibody

Mouse Monoclonal RPA-T4 IgG1 kappa
Code

200-302-M63
Size

500 µL
Price

$145.00
Availability

Ships within 2 weeks.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Cell Surface Flow Cytometry of Mouse anti-HUMAN CD4 antibody Fluorescein conjugated.  
Cells:  10^6 human PBMC.  Propidium iodide negative lymphocyte population gated for analysis.
Stimulation: none.
Antibody:  (GREEN) isotype control antibody; (RED) Fluorescein Anti-CD4 mouse secondary antibody using 10 ul (0.1 ug).
Shipping & Handling Fees Apply.

Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4, FITC anti-human CD4, RPA-T4, FITC, T4

CD4 Fluorescein Antibody Properties

Description
Anti-CD4 (MOUSE) Fluorescein Conjugated Monoclonal Antibody - 200-302-M63
Host
Mouse
Target Species
Human
Known Cross Reactivity
human
Clonality
Monoclonal RPA-T4 IgG1 kappa
Application
IF Microscopy : User Optimized
FlowCytometry : 10 µL/10^6 cells (0.1 µg)
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG1
FP Value
2-8 moles Fluorescein (FITC) per mole of IgG1
Concentration
0.2mg/mL Sufficient to run approximately 100 tests
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.09% (w/v) Sodium Azide
Stabilizer
0.1% Gelatin
GeneName
CD4
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Lot#  

CD4 Fluorescein Antibody Description

Background
CD4 is an Immunoglobulin Superfamily protein and an accessory protein for MHC class-II antigen/ T-cell receptor interaction. It may regulate T-cell activation. It plays a more general role in mediating cell recognition events than merely those of cellular immune response. The T4 molecule serves as a receptor for the human immunodeficiency virus. A Type I membrane protein, it is expressed on T lymphocytes, B-cells, macrophages, and granulocytes. It is also expressed in a developmentally regulated manner in specific regions of the brain.
Immunogen
Anti-CD4 Antibody (Monoclonal) was produced by repeated immunizations with PHA-stimulated human peripheral blood mononuclear cells (PBMC).
Immunogen Type
Other
Storage Condition
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
Application Note
Anti-CD4 is useful for Immunofluorescence and Flow Cytometry (Cell Surface). Researchers should determine optimal titers for applications that are not stated.
Purity/Specifity
Fluorescein conjugated CD4 Monoclonal Antibody was Protein G Purified from tissue culture supernatant and is directed against human CD4. Reactivity is observed against human CD4 and chimpanzee. Cross reactivity with CD4 from other sources has not been tested.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
1. Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York. Toma J, Weinheimer SP, Stawiski E, Whitcomb JM, Lewis ST, Petropoulos CJ, and Huang W. 2011. J. Virol. 85: 3872-3880. Porter KA, Kelley LN, Nekorchuk MD, Jones JH, Hahn AB, de Noronha CMC, Harton JA, and Duus KM. 2010. J. Immunol. 185:6480-6488. Hsieh S-C, Tsai W-Y, and Wang W-K. 2010. J. Virol. 84(9). : 4782-4797. Chen X, Wang X, Besra GS, and Gumperz JE. 2007. J. Leukoc. Biol. 82:1455-1465. Thedrez A, de Lalla C, Allain S, Zaccagnino L, et al. 2007. Blood. 110:251-258 Mack CL, Tucker RM, Sokol RJ, Darrer FM, Kotzin BL, Whitington PF and Miller SD. 2004. Pediatr. Res. 56(1). :79-87. Deng MC, Bell S, Huie P, Pinto F, Hunt SA, Stinson EB, Sibley R, Hall BM, and Valantine HA. 1995. Circulation. 91: 1647-1654.
Specific Reference
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Product Type
Proteins and Peptides;
Applications
ELISA, FLISA, WB, IF, FC
Catalog Number
010-001-330

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human, chimpanzee
Catalog Number
200-401-908

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-10

Product Type
Molecular Biology Reagents;
Catalog Number
MB-008

Product Label

 

Description
Fluorescein (FITC)
Color
Green
Conjugation Reference
Modified from The and Feltkamp, Immunology 18; 865, 1970.
Molecular Weight
390
Excitation Wavelength
495
Conjugation Chemistry
Isothiocyanate