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CD3e Fluorescein Antibody

Hamster Armenian Monoclonal 145-2C11 IgG
NCI Collaboration
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Cell Surface Flow Cytometry of Hamster anti-MOUSE CD3 antibody Fluorescein conjugated.  
Cells: 10^6 BALB/c mouse splenocytes. 
Stimulation: none.
Antibody:  (GREEN) isotype control antibody;   (RED) Fluorescein Anti-CD3 Hamster secondary antibody using 0.25 µg.
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Number:
200-B02-M69
Size:
100 µg
Price:
$105.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships within 2 weeks.

CD3e Fluorescein Antibody Properties

Description
Anti-CD3 epsilon (ARMENIAN HAMSTER) Fluorescein Conjugated Monoclonal Antibody - 200-B02-M69
Host
Hamster Armenian
Target Species
Mouse
Known Cross Reactivity
Mouse
Clonality
Monoclonal 145-2C11 IgG
Application
Flow Cytometry : 0.1-0.5 µg/10^6 cells
Other Dilution: Sufficient to run approximately 500 tests
Physical State
Liquid (sterile filtered)
Shipping Condition
Wet Ice
Format
IgG
FP Value
2-8 moles Fluorescein (FITC) per mole of IgG
Concentration
0.5mg/mL by UV absorbance at 280 nm
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.09% (w/v) Sodium Azide
Stabilizer
0.1% Gelatin
GeneName
Cd3e
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CD3e Fluorescein Antibody Description

Background
CD3 is a multi-subunit complex consisting of 4 different invariable membrane proteins, CD3E, CD3D, CD3G and CD3-omega. These CD3 proteins are expressed on the surface of T-cells and Thymocytes during thymocyte development. They are non-covalently associated with the TCR heterodimer and form the functional TCR-CD3 antigen receptor complex of the T-lymphocyte. CD3 expression is significant during Intrathymic T-lymphocyte maturation, TCR signaling and Thymocyte differentiation. Stimulation of TCR-CD3 complex results in activation of various protein tyrosine kinases including ZAP-70 affecting multiple cellular responses including T-cell proliferation, clonal expansion, induction of tolerance and apoptosis. Mutations in the sub-unit chains of CD3 have been associated with various immunodeficiency syndromes including SCID.
Synonyms
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e
Immunogen
Anti-CD3 Antibody (Monoclonal) was produced by repeated immunizations with H-2Kb specific cytotoxic T lymphocyte clone BM10-37.
Immunogen Type
Other
Storage Condition
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
Application Note
Anti-CD3 is useful for Flow Cytometry (Cell Surface). Researchers should determine optimal titers for applications that are not stated.
Purity/Specifity
Fluorescein conjugated CD3 Monoclonal Antibody was Protein G Purified from tissue culture supernatant and is directed against mouse CD3. Reactivity is observed against mouse CD3. Cross reactivity with CD3 from other sources has not been tested.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Staehli R, Ludigs K, Heinz LX, Sequin-Estevez Q, Ferero I, Braun M, Schroder K, Rebsamen M, Tardivel A, Mattmann C, MacDonald HR, Romero P, Reith W, Guarda G, and Tschopp J. 2012. J. Immunol. 188: 3820-3828. Todo T, Wu G, Chai NN, He Y, Martins G, Gupta A, Fair J, Liu NY, Jordan S, and Klein A. 2012. Int. Immunol. 10:1093. Mira E, Leon B, Barber DF, Jimenez-Baranda S, Goya I, Almonacid L, Marquez G, Zaballos A, Martinez C, Stein JV, Ardavin C and Manes S. 2012. J. Immunol. Becker-Herman A, Meyer-Bahlburg A, Schwartz MA, Jackson SW, Hudkins KL, Liu C, Sather BD, Khim S, Liggitt D, Song W, Silverman GJ, Alpers CE and Rawlings DJ. 2011. J. Exp. Med. 208:2033-2042. Salmond RJ, Filby A, Pirinen N, Magee AI, and Zamoyska R. 2010. Blood. 117: 108-117. Tilley SL, Jaradat M, Stapleton C, Dixon D, Hua X, Erikson CJ,. McCaskill JG, Chason KD, Liao G, Jania L, Koller BH, and Jetten AM. 2007. J. Immunol. 178: 3208-18. Isakov N, Wange RL, Burgess WH, Watts JD, Aebersold R, and Samelson LE. 1995. J. Exp. Med. 181:375-380. Salvadori S, Gansbacher B, Pizzimenti AM, and Zier KS. 1994. J. Immunol. 153: 5176 - 5182. Leo O, Foo M, Sachs D, Samuelson L, and Bluestone J. 1987. Proc. Natl. Acad. Sci. USA. 84: 1374
Specific Reference
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Product Type
Proteins and Peptides;
Applications
FLISA, IF, FC
Catalog Number
019-001-002

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human, chimpanzee
Catalog Number
200-401-908

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-10

Product Type
Molecular Biology Reagents;
Applications
ELISA, WB
Catalog Number
MB-008

Product Label

 

Description
Fluorescein (FITC)
Color
Green
Conjugation Reference
Modified from The and Feltkamp, Immunology 18; 865, 1970.
Molecular Weight
390
Excitation Wavelength
495
Conjugation Chemistry
Isothiocyanate