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Anti-RON pY1353 (RABBIT) Antibody - 600-401-G69
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Primary Antibodies  >  Cell Cycle Protein Antibodies
 

RON pY1360 Antibody

Rabbit Polyclonal
Code

600-401-G70
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Western Blot of Rabbit anti-RONpY1360 antibody.  
Lane 1:  Phospho RON rProtein (cytoplasmic domain).  
Lane 2:  Phospho RON rProtein (cytoplasmic domain) incubated with RONpY1360 peptide. 
Load:  0.05 µg per lane.
Primary antibody:  RON pY1360 antibody at 1µg for overnight at 4°C.
Secondary antibody:  HRP Goat anti-rabbit IgG secondary antibody at 1:40,000 for 45 min at RT.
Block:  MB-070 Fluorescent blocking buffer overnight at 4°C.
Predicted/Observed size:  152 kDa (full legnth) /~75 kDa (cytoplasmic domain) RON pY1360. 
Other band(s): none.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Macrophage-stimulating protein receptor, p185-Ron, PTK8, RON, Protein-tyrosine kinase 8, c-met-related tyrosine kinase

RON pY1360 Antibody Properties

Description
Anti-RON pY1360 (RABBIT) Antibody - 600-401-G70
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:2,000 - 1:10,000
Western Blot : 1:500-1:2000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.79 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
MST1R
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Lot#  

RON pY1360 Antibody Description

Background
RON pY1360 antibody detects phosphorylated RON. RON is a member of the protein kinase superfamily. Protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. RON is a heterodimer composed of an a chain and a ß chain. The a chain is completely extracellular, whereas the ß chain traverses the cell membrane and contains the intracellular tyrosine kinase and regulatory elements. Anti-RON Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.
Immunogen
Anti-RON pY1360 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y1360 of human RON protein.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-RONpY1360 Antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 152.3 kDa corresponding to full length protein by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with human RON pY1360 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Purity/Specifity
Anti-RON pY1360 was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorptions against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for human RON protein phosphorylated at Y1360. Cross-reactivity against RON from other species may occur but has not yet been tested.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Chaudhuri A, Xie MH, Yang B, Mahapatra K, Liu J, Marsters S, Bodepudi S, and Ashkenazi A. (2011) Distinct Involvement of the Gab1 and Grb2 Adaptor Proteins in Signal Transduction by the Related Receptor Tyrosine Kinases RON and MET. The Journal of Biological Chemistry. VOL. 286, NO. 37, pp. 32762–32774.
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
600-401-G69

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry