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Datasheet

RON phospho Y1360 Antibody

Rabbit Polyclonal

600-401-G70S 600-401-G70
25 µL 100 µg
WB, ELISA
Human
Rabbit
$125.00 /Per Item
$475.00 /Per Item
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Background

RON pY1360 antibody detects phosphorylated RON. RON is a member of the protein kinase superfamily. Protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. RON is a heterodimer composed of an α chain and a β chain. The α chain is completely extracellular, whereas the β chain traverses the cell membrane and contains the intracellular tyrosine kinase and regulatory elements. Anti-RON Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.

Product Details

Anti-RON pY1360 (RABBIT) Antibody - 600-401-G70
rabbit anti-RON pY1360 antibody, Macrophage-stimulating protein receptor, MSP receptor, Protein-tyrosine kinase 8, p185-Ron, PTK8, RON, c-met-related tyrosine kinase, CD136
Rabbit
Polyclonal
IgG

Target Details

MST1R  - View All MST1R Products
Human
Phosphorylation
Conjugated Peptide
Anti-RON pY1360 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y1360 of human RON protein.
Anti-RON pY1360 was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorptions against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for human RON protein phosphorylated at Y1360. Cross-reactivity against RON from other species may occur but has not yet been tested.

Application Details

ELISA, WB
Anti-RONpY1360 Antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 152.3 kDa corresponding to full length protein by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with human RON pY1360 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Formulation

Liquid (sterile filtered)
0.79 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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