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Primary Antibodies  >  Enzyme Antibodies
 

Anti-GAB1 Antibody

Rabbit Polyclonal
Code

600-401-K05
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western Blot of Rabbit anti-GAB1 antibody.  
Lane 1:  Phospho GAB1 rProtein.  
Lane 2:  Phospho GAB1 rProtein incubated with GAB1 peptide.  
Load:  0.05 µg per lane.
Primary antibody:  GAB1 antibody at 1µg/mL for overnight at 4°C.
Secondary antibody:  HRP Goat anti-Rabbit IgG secondary antibody at 1:40,000 for 45 min at RT.
Block:  MB-070 Fluorescent blocking buffer overnight at 4°C.
Predicted/Observed size:  130 kDa for GAB1. 
Other band(s): none.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: GRB2-associated-binding protein 1; GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1

Anti-GAB1 Antibody Properties

Description
Anti-GAB1 (RABBIT) Antibody - 600-401-K05
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:2,000 - 1:10,000
Western Blot : 1:500-1:2000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.62 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
GAB1
Related Links
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Lot#  

Anti-GAB1 Antibody Description

Background
GAB1 antibody detects human GAB1. GAB1 is a member of the IRS1-like multisubstrate docking protein family. The protein is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. GAB1 plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. It is known to play a role in FGFR1 signaling and is probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). GAB1 interacts with GRB2 and with other SH2-containing proteins. It is known to interact with phosphorylated LAT2, PTPRJ, FRS2, GRB2, PIK3R1 and SOS1. GAB1 gets phosphorylated in response to FGFR1 activation. This tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. Anti-GAB1 Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.
Immunogen
Anti-GAB1 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y659 of human GAB1 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-GAB1 Antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at 76.6 kDa by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
Anti-GAB1 was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorptions against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for human GAB1. Cross-reactivity against GAB1 from other species may occur but has not yet been tested.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Chaudhuri A, Xie MH, Yang B, Mahapatra K, Liu J, Marsters S, Bodepudi S, and Ashkenazi A. (2011) Distinct Involvement of the Gab1 and Grb2 Adaptor Proteins in Signal Transduction by the Related Receptor Tyrosine Kinases RON and MET. The Journal of Biological Chemistry. VOL. 286, NO. 37, pp. 32762–32774.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-269

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
600-401-897

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry