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GFP and Epitopes  >  Antibodies to GFP and Antibodies to RFP
 

RFP Antibody Biotin Conjugated

Chicken Polyclonal
Code

600-906-379
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot of Rockland's Anti-RFP Antibody. 0.1 µg of RFP was loaded on a 4-20% gel and tranferred to nitrocellulose membrane. Anti-RFP Antibody was added at 1.0 µg/mL at RT for 2 hours. IRDye800® anti-Chicken (p/n 603-132-126) was added at 1:20,000 at RT for 45 minutes.
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Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

RFP Antibody Biotin Conjugated Properties

Description
Anti-RFP (CHICKEN) Biotin Conjugated Antibody - 600-906-379
Host
Chicken
Clonality
Polyclonal
Application
ELISA : 1:10,000
Western Blot : 1:1,000 - 1:3,000
Other Dilution: User Optimized
Epitope Tag Type
RFP
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
GeneName
DsRed
Related Links
Download CofA
Lot#  

RFP Antibody Biotin Conjugated Description

Background
Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including western blotting.
Immunogen
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-RFP is designed to detect recombinant RFP. This antibody can be used to detect RFP by immunoblotting. Use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.
Purity/Specifity
RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above.  RFP Antibody was tested by western blot and immunoelectrophoresis.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Gross LA, Baird GS, Hoffman RC, Baldridge KK, Tsien RY: The structure of the chromophore within DsRed, a red fluorescent protein from coral. Proc. Natl. Acad. Sci. USA 2000, 97: 11990-11995.
Specific Reference
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Product Type
GFP and Epitopes;
Catalog Number
000-001-379

Product Type
Proteins and Peptides;
Catalog Number
003-0202

Product Type
GFP and Epitopes;
Applications
ELISA, WB
Catalog Number
600-901-379S

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Chicken
Catalog Number
603-103-002

Product Label

 

Description
Biotin
Color
n/a
Conjugation Reference
Modified from Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
Molecular Weight
455
Excitation Wavelength
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester