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Primary Antibodies  >  Transcription Factor Antibodies
 

TLR9 Antibody

Mouse Monoclonal 26C593.2 IgG1 kappa
Code

200-301-I31
Size

100 µg
Price

$309.00
Availability

Ships within 2 weeks.
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Flow Cytometry of Mouse Anti-TLR9 antibody.  
Cells:  human PBMC
Stimulation:  none.
Primary Antibody: Anti-TLR9 antibody at 0.5 ug (red) and  isotype control (green).                                                            Secondary Antibody:
Immunohistochemistry of mouse Anti-TLR9 CD288 antibody.  
Tissue A:  Adenocarcinoma of the lung. Tissue C:  A549 cells. Tissue F:  malignant lung. 
Fixation:  formalin fixed paraffin embedded.  
Antigen retrieval:  not required.
Primary antibody: TLR9 antibody at 1:100 for 1 h at RT. 
Secondary antibody:  Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT.                                   Localization:  TLR9 is an endoplasmic reticulum membrane antibody and is a single-pass type 1 membrane protein.                                
Staining:
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Shipping:
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Synonyms: Toll-like receptor 9

TLR9 Antibody Properties

Description
Anti-TLR9 (MOUSE) Monoclonal Antibody - 200-301-I31
Host
Mouse
Target Species
Human
Known Cross Reactivity
Human, Mouse, Rat
Clonality
Monoclonal 26C593.2 IgG1 kappa
Application
FlowCytometry : 0.1-2 µg/10^6 cells
Western Blot : 1-5 µg/mL
Immunochemistry: 10-20 µg/mL (frozen)
Physical State
Liquid
Shipping condition
Dry Ice
Format
IgG1
Label
Concentration
0.5 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.05% (w/v) Sodium Azide
Stabilizer
0.05% BSA
GeneName
TLR9
Download CofA
Lot#  

TLR9 Antibody Description

Background
Anti-TLR9 CD289 Antibody detects human TLR9 CD289. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. By using a BLAST search, a cDNA coding for TLR9 has been identified and subsequently isolated. Gene knockout experiments suggest that TLR9 acts as a receptor for unmethylated CpG dinucleotides in the bacterial DNA. Human and mouse TLR9 share an overall amino-acid identity of 75.5%. TLR9 is highly expressed in spleen. Anti-TLR9 CD289 Antibody is ideal for investigators involved in cytokines and growth factor research.
Immunogen
TLR9 Antibody was produced in mice prepared repeated immunizations with amino acids corresponding to an internal sequence of human TLR9 isoform A protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-TLR9 Antibody is suitable for use inCM, Flow (CS), Flow (I), IF/ICC, IHC (frozen), IHC (p) and WB. Expect a band approximately 113kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-TLR9 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-TLR9 from monkey based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-TLR9 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
1. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. Exp. Med. 187: 2097-2101 (1998). 2. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997). 3. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2): 157-161 (2001). 4. Du X, Poltorak A, Wei Y, Beutler B. Eur Cytokine Netw 11(3): 362-71 (2000). 5. Chuang TH and Ulevitch RJ. Eur Cytokine Netw 11(3): 372-378 (2000).
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-1302

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry