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HDAC-7 (N-terminus) Antibody

Rabbit Polyclonal
NCI Collaboration
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Western Blot of Rabbit anti-HDAC7 antibody.  
Lane 1:  mouse brain homogenate.  
Lane 2:  mouse brain homogenate blocked with peptide.  
Load:  4 µg per lane.
Primary antibody:  HDAC7 antibody at 1µg/mL for overnight at 4°C.
Secondary antibody:  HRP rabbit secondary antibody at 1:40,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  ~103kDa/ ~103, 70, and 40kDa for HDAC7. 
Other band(s): HDAC7 isoforms ~70 and 40kDa.
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Number:
600-401-J22
Size:
100 µg
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

HDAC-7 (N-terminus) Antibody Properties

Description
Anti-HDAC-7 (RABBIT) Antibody - 600-401-J22
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human, Mouse, Rat, Primate
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:60,000
IF Microscopy : 1:100-1:500
Western Blot : 1 µg/ml
Immunohistochemistry: 1:100-1:500
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
1.16 mg/ml by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
HDAC7
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HDAC-7 (N-terminus) Antibody Description

Background
HDAC7 is a member of the class II mammalian histone deacetylases, which plays an important role in modulating the eukaryotic chromatin structure. Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm, suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a carboxy-terminal domain and is dependent on interaction with class I HDACs in the nucleus. It is an active component of different transcriptional corepressor complexes that can be recruited to specific promoter regions via interactions with a growing number of sequence specific transcriptional factors. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions, leading to the inhibition of gene transcription. Anti-HDAC7 antibodies are ideal for researches interested in Breast Cancer, Cancer, Cell Cycle and Replication, Chromatin Research, Epigenetics, and Histone Deacetylases research.
Synonyms
DKFZP586J0917, EC 3.5.1.98, HD7, HD7A, HD7a, HDAC7AFLJ99588, histone deacetylase 7, Histone deacetylase 7ADKFZp586J0917
Immunogen
HDAC7 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N terminal region of human HDAC7.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-HDAC7 antibody is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~103kDa and isoforms at ~70, and 40 kDa corresponding to the appropriate cell lysate or extract.
Purity/Specifity
Anti-HDAC7 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards HDAC7. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, Rat and Primate based on 100% sequence homology. Cross-reactivity with HDAC7 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Annemieke J.M. de Ruijter, Albert H. van Gennip, Huib N. Caron, Stephan Kemp, André B.P. van Kuilenburg,. Histone Deacetylases Characterisation of the Classical HDAC FamilyLY, Biochemical Journal Immediate Publication. Published on 12 Nov 2002 as manuscript BJ20021321. [PMID: 12429021] Lee, H J; Chun M, Kandror K V. Tip60 and HDAC7 interact with the endothelin receptor a and may be involved in downstream signaling. J. Biol. Chem. 276 (20): 16597–600, . 2001. [PMID: 11262386]
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human
Catalog Number
600-401-879

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Molecular Biology Reagents;
Applications
ELISA
Catalog Number
MB-064-1000

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry