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Primary Antibodies  >  Neuroscience Antibodies
 

Beta Amyloid pyro E11 Antibody

Rabbit Polyclonal
Code

600-401-J13
Size

50 µg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Western Blot of Rabbit Anti-beta Amyloid pyro E11 antibody.  
Lane 1:  Ovalbumin Control.  
Lane 2:  beta Amyloid pyro E11 peptide.  Lane 3:  beta Amyloid 11 peptide.  
Lane 4:  beta Amyloid pyro E3 peptide.  
Load:  1 µg per lane.
Primary antibody:  ß Amyloid pyro E11 antibody at 0.2µg/mL for overnight at 4°C.
Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  86.9 kDa for beta Amyloid pyro E11 peptide.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Abeta, Beta Amyloid, beta-amyloid peptide, Beta Amyloid Antibody

Beta Amyloid pyro E11 Antibody Properties

Description
Anti-Beta Amyloid pyro E11 (RABBIT) Antibody - 600-401-J13
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human, Primate
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:60,000
IF Microscopy : 1:100-1:500
Western Blot : 0.2 µg/ml
Immunochemistry: 1:100-1:500
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
1 mg/ml by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL to 20 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
GeneName
APP
Download CofA
Lot#  

Beta Amyloid pyro E11 Antibody Description

Background
The cerebral and vascular plaques associated with Alzheimer's disease are mainly composed of Amyloid beta peptides. Beta Amyloid is derived from cleavage of the Amyloid precursor protein and varies in length from 39 to 43 amino acids. Beta Amyloid [1-40], beta Amyloid [1-42], and beta Amyloid [1-43] peptides result from cleavage of Amyloid precursor protein after residues 40, 42, and 43, respectively. The cleavage takes place by gamma-secretase during the last Amyloid precursor protein processing step. Beta Amyloid [1-40], beta Amyloid [1-42], and beta Amyloid [1-43] peptides are major constituents of the plaques and tangles that occur in Alzheimer's disease. Beta Amyloid antibodies and peptides have been developed as tools for elucidating the biology of Alzheimer's disease. Anti-beta Amyloid [Pyro Glu11] antibody is ideal for researchers interested Alzheimers Research, Cell Cycle and Replication, and Neuroscience research.
Immunogen
beta Amyloid [Pyro Glu11] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-terminus 11-pyro E start point of human beta Amyloid.
Immunogen Type
Peptide
Post Translational Modification
Pyroglutamate
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Beta Amyloid pyro Glu11 antibody is useful for ELISA, Western Blot and Immunostaining. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~86.9kDa corresponding to the appropriate cell lysate or extract.
Purity/Specifity
Anti-Beta Amyloid pyro Glu11 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific to the N-terminus 11-pyro E start point. A BLAST analysis was used to suggest cross-reactivity with Human and Primate based on 100% sequence homology. Cross-reactivity with beta Amyloid pyro Glu11 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Youssef I, Florent-Béchard S, Malaplate-Armand C, Koziel V, Bihain B, Olivier JL, Leininger-Muller B, Kriem B, Oster T, Pillot T.,. N-truncated amyloid-beta oligomers induce learning impairment and neuronal apoptosis. Neurobiology of Aging 29 1319–1333. 2008. [PMID: 17459527] Schilling S, Lauber T, Schaupp M, Manhart S, Scheel E, Böhm G, Demuth HU. On the seeding and oligomerization of pGlu-amyloid peptides (in vitro). Biochemistry;45(41):12393-9. 2006 Oct 17. [PMID: 17029395]
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse
Catalog Number
600-401-253

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Molecular Biology Reagents;
Applications
ELISA
Catalog Number
MB-064-1000

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry