14-10_house_marker_thin
 
You may also want to consider these related products.
Anti-HDAC-1 (RABBIT) Antibody - 600-401-879
Anti-HDAC-1 (RABBIT) Antibody - 600-401-879
BOVINE SERUM ALBUMIN 30% Solution - BSA-30
BOVINE SERUM ALBUMIN 30% Solution - BSA-30
ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free) - MB-064-1000
ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free) - MB-064-1000
10X HDAC Assay Buffer - MB-068-0020
10X HDAC Assay Buffer - MB-068-0020
Primary Antibodies  >  Epigenetic Research Antibodies
 

HDAC-4 (internal) Antibody

Rabbit Polyclonal
Code

600-401-J20
Size

100 µg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Western Blot of Rabbit anti-HDAC4 antibody.  
Lane 1:  Mouse brain homogenate.  
Lane 2:  Mouse brain homogenate blocked with peptide.  
Load:  30 µg per lane.
Primary antibody:  HDAC4 antibody at 1:400 for overnight at 4°C.
Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  140 kDa, 98 kDa,  and 55 kDa for HDAC4. 
Other band(s): HDAC4 splice variants and isoforms.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: HA6116, HDAC-A, HDACABDMR, HD4EC 3.5.1.98, histone deacetylase A, histone deacetylase 4, KIAA0288AHO3

HDAC-4 (internal) Antibody Properties

Description
Anti-HDAC-4 (RABBIT) Antibody - 600-401-J20
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human, Mouse, Rat, Primate
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:60,000
IF Microscopy : 1:100-1:500
Western Blot : 1 mg/mL
Immunochemistry: 1 mg/mL
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer
50% (v/v) Glycerol
GeneName
HDAC4
Download CofA
Lot#  

HDAC-4 (internal) Antibody Description

Background
HDAC4 is a 140 kD class II histone deacetylase similar to the yeast HDA1 protein. This nuclear protein operates in concert with histone acetyltransferases to control core histone acetylation in highly conserved lysine residues. HDAC activity has been associated with transcriptional repression and nucleosomal condensation. HDAC4 uses active nuclear export to shuttle between the nucleus and the cytoplasm. HDAC4 is modified by phosphorylation by CaM kinase I or IV. HDAC4 has been reported to interact with 14-3-3 in the cytoplasm and MEF2A, HDAC3, NcoR and SMRT in the nucleus. Anti-HDAC4 antibodies are ideal for researchers interested in Breast Cancer, Cancer, Cell Cycle and Replication, Chromatin Research, Epigenetics, and Histone Deacetylases research.
Immunogen
HDAC4 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region surrounding 600-650aa of human HDAC4.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-HDAC4 antibody is useful for ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~140 kDa corresponding to the appropriate cell lysate or extract.
Purity/Specifity
Anti-HDAC4 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards HDAC4. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, Rat, and Primate based on 100% sequence homology. Cross-reactivity with HDAC4 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Bertos NR, Wang AH, Yang XJ. (2001) Class II histone deacetylases: structure, function, and regulation. Biochem Cell Biol;79(3):243-52. [PMID: 11467738] Chen B, Cepko CL. (2009) HDAC4 regulates neuronal survival in normal and diseased retinas. Science; 323(5911):256-9. Jan 9. [PMID: 19131628].
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human
Catalog Number
600-401-879

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Molecular Biology Reagents;
Applications
ELISA
Catalog Number
MB-064-1000

Product Type
Molecular Biology Reagents;
Catalog Number
MB-068-0020

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry