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Anti-Low Molecular Weight Proteins Host Cell Protein (HCP) Antibody - 100-401-J06
Anti-Low Molecular Weight Proteins Host Cell Protein (HCP) Antibody - 100-401-J06
Kits  >  Loading Control Antibodies
 

E.coli HCP Antibody

Rabbit Polyclonal
Code

100-401-J07
Size

100 µL
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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2D Western Blot of anti-E.coli HCP antibody.  
Load: 35 µg Total HCP.
pH: 5-8.
Primary antibody: Rabbit anti-HCP antibody at 1:200 for overnight at 4°C.
Secondary antibody:  Goat anti-rabbit secondary antibody at 1:10,000 for 30 min at RT.
Block:  MB-070 for 1 hour at RT.
Western Blot of Rabbit anti-HCP antibody.  
Lane 1:  Molecular Weight.  
Lane 2:  Total HCP.  
Load:  10µg per lane.
Primary antibody:  Rabbit anti-HCP cocktail at 1:1000 for overnight at 4°C.
Secondary antibody:  Goat anti-rabbit secondary antibody at 1:10,000 for 30 min at RT.
Block:  MB-070 for 1 hour at RT.
Shipping & Handling Fees Apply.

Shipping:
$20.00 to United States
Handling:
$20.00
Synonyms: Host Cell Protein antibody, HCP antibody, Anti-HCP, Anti-Host Cell Protein, ECP antibody, Anti-ECP

E.coli HCP Antibody Properties

Description
Anti-Host Cell Protein (HCP) (RABBIT) Antibody - 100-401-J07
Host
Rabbit
Target Species
Escherichia coli
Known Cross Reactivity
E.coli
Clonality
Polyclonal
Application
ELISA : 1:1000-1:2000
Western Blot : 1:500 - 1:1000
Other Dilution: User Optimized
Product Type
Component
Kit Type
Chemiluminescent Western Blot Kit
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
77.5 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
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Lot#  

E.coli HCP Antibody Description

Background
Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli. This antibody has been validated by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
Immunogen
Anti-Host Cell Proteins Antibody was prepared combining antibodies that have been separatly developed by repeated immunizations with a mixture of high molecular weight (HMW) and a mixture of low molecular weight (LMW) proteins from E.Coli.
Immunogen Type
Native Protein
Storage Condition
Store Host Cell Proteins Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Host Cell Proteins Antibody detects over 300 E.coli proteins and is suitable for western blotting and ELISA.
Purity/Specifity
This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. Reactivity is directed against E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50


Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
E.coli
Catalog Number
100-401-J06

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry