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Primary Antibodies  >  Epigenetic Research Antibodies
 

Histone H3 K4me2 Antibody

Rabbit Polyclonal
Code

600-401-I58
Size

50 µg
Price

$345.00
Availability

Ships next business day.
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Immunofluorescence of Rabbit Anti-Histone H3 [Dimethyl Lys4]  Antibody.  
Tissue:  C. elegans embryo lysate.
Fixation:  0.5% PFA. 
Antigen retrieval:  Not required.
Primary antibody:  Histone H3 [Dimethyl Lys4] antibody at a 1:50 dilution for 1 h at RT.
Secondary antibody:  Dylight 488 secondary antibody at 1:10,000 for 45 min at RT.
Localization:  Histone H3 [Dimethyl Lys4] is nuclear and chromosomal.
Staining:  Histone H3 [Dimethyl Lys4] is expressed in green and the nuclei are counterstained blue with DAPI.
Western Blot of Rabbit Anti-Histone H3 [Dimethyl Lys4] Antibody.
Lane 1:  HeLa histone preps.
Load:  30 µg per lane.
Primary antibody: Histone H3 [Dimethyl Lys4] at 1:1000 for overnight at 4°C.
Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  ~15 kDa.
Other band(s): None.
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Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: H3.3B, H3.3AH3F3H3F3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3K4me2

Histone H3 K4me2 Antibody Properties

Description
Anti-Histone H3 [Dimethyl Lys4] (RABBIT) Antibody - 600-401-I58
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
Clonality
Polyclonal
Application
ChIP : 2-5µg/million cells
IF Microscopy : 1:50
Western Blot : 1:500
Immunochemistry: 1:50
Other Dilution: Dot Blot 1:1000
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.7 mg/ml by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.05% (w/v) Sodium Azide
Stabilizer
30% Glycerol
GeneName
HIST2H3C
Download CofA
Lot#  

Histone H3 K4me2 Antibody Description

Background
H3K4me2 is a modification thought to have a role in transcriptional memory. In CD4+ T lymphocytes, H3K4Me2 is present within gene bodies regulating cellular function, but not in those of housekeeping genes, which indicates that the modification has a role in refining the tissue-specificity of expressed genes. This type of cellular identity targeting is also noted in work with human and mouse spermatozoa; the H3K4Me2 modification marks genes that are relevant in spermatogenesis. Most effects of H3K4Me2 seem to be attributed to its transcriptional activation; however, recent work also indicates that it may also play an RNA-dependent regressive role, related to the GAL-1 promoter. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics Research.
Immunogen
Histone H3 [Dimethyl Lys4] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 4 of human Histone H3.2.
Immunogen Type
Peptide
Post Translational Modification
Methylation
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Histone H3 [Dimethyl Lys4] antibody is useful for Western Blot, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
Purity/Specifity
Anti-Histone H3 [Dimethyl Lys4] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Campsteijn C., Øvrebø JI., Karlsen BO., Thompson EM. (2012). Expansion of cyclin D and CDK1 paralogs in Oikopleura dioica, a chordate employing diverse cell cycle variants. Mol. Biol. Evol. 2012 Feb. 29:487–502. Yue C., Soboloff J., Gamero AM. (2012). Control of type I interferon-induced cell seath by Orai1-mediated calcium entry in T cells. J Biol Chem. 2012 Jan. 287:3207–3216. Kung VL., Khare S., Stehlik C., Bacon EM., Hughes AJ., Hauser AR. (2012). An rhs gene of Pseudomonas aeruginosa encodes a virulence protein that activates the inflammasome. Proc Natl Acad Sci USA. 2012 Jan. 109:1275–1280. Sullivan CP., Berg EA., Elliot-Bryant R., Fishman JB., Mckee AC., Morin PJ., et al. (2011). Pyroglutamate-?ß 3 and 11 colocalize in amyloid plaques in Alzheimer's disease cerebral cortex with pyroglutamate-?ß 11 forming the central core. Neurosci Lett. 2011 Nov 14. 505(2):109-12. Daftarian P., Kaifer AE., Li W., Blomberg BB., Frasca D., Roth F., et al. (2011). Peptide-conjugated PAMAM dendrimer as a universal platform for antigen presenting cell targeting and effective DNA-based vaccinations. Cancer Res. 2011 Oct 10. 71(24):7452-62.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Drosophila melanogaster
Catalog Number
600-401-914

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry