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Bovine Serum Albumin Antibody BSA

Rabbit Polyclonal
NCI Collaboration
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Western blot of Rabbit Anti-Bovine Serum Albumin secondary antibody.
Lane 1:  BSA.
Lane 2:  None.
Load:  50 ng per lane.
Primary antibody:  BSA antibody at 1:1,000 overnight at 4°C.
Secondary antibody:  Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.
Blocking:  5% Goat Serum for 30 min at RT.
Predicted/Observed size:  66 kDa, 61 kDa for BSA.
Other band(s):  None.
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Number:
201-4133-0100
Size:
100 µg
Price:
$309.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

Bovine Serum Albumin Antibody BSA Properties

Description
Anti-BOVINE SERUM ALBUMIN (RABBIT) Antibody - 201-4133-0100
Host
Rabbit
Target Species
Bovine
Known Cross Reactivity
bovine
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000-1:10,000
Immunohistochemistry: 1:1,000 - 1:5,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping Condition
Ambient
Format
IgG
Label
Concentration
1 mg/ml by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
ALB
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Bovine Serum Albumin Antibody BSA Description

Background
Bovine Serum Albumin antibody detects BSA. Serum albumin is the main protein of plasma. It has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. ALB is the major zinc transporter in plasma; it typically binds about 80% of all plasma zinc. BSA antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.
Synonyms
ALB antibody, Albumin antibody, Allergen Bos d 6 antibody, BSA antibody, DKFZp779N1935 antibody, Anti-BSA
Immunogen
Anti-Bovine Serum Albumin antibody was produced by repeated immunizations with bovine serum albumin.
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-ALB antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
Purity/Specifity
Bovine Serum Albumin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Albumin (Bovine Serum).  Cross reactivity against Albumin from other tissues and species may occur but has not been specifically determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
bovine
Catalog Number
201-301-033

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-401-033

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
mouse
Catalog Number
600-401-254

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry