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GFP and Epitopes  >  Antibodies to GFP and Antibodies to RFP
 

RFP Antibody

Mouse Monoclonal 8E5.G7 IgG2a kappa
NCI Collaboration
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Western Blot of Mouse Anti-RFP antibody.  
Lane 1:  YFP protein.
Lane 2:  Prestained Molecular Weight Marker. 
Lane 3:  Reduced RFP control Protein.
Lane 4:  Reduced mCherry.
Lane 5:  GFP potein.
Lane 6:  Non-Reduced RFP conteol Protein.
Lane 7:  Non-Reduced mCherry.
Load:  300ng per lane.
Primary antibody:  RFP antibody at 1:2000 in MB-070 for 3 hours at RT.
Secondary antibody:  HRP anti-Mouse secondary antibody at 1:10,000 in MB-070 for 60 min at RT.  Substrate: TMBM-100 for 20 min.
Predicted/Observed size:  ~27 kDa.
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Number:
200-301-379
Size:
100 µg
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

RFP Antibody Properties

Description
Anti-RFP (MOUSE) Monoclonal Antibody - 200-301-379
Host
Mouse
Known Cross Reactivity
Red Fluorescent Protein (RFP)
Clonality
Monoclonal 8E5.G7 IgG2a kappa
Application
ELISA : 1:20,000-1:50,000
IF Microscopy : User Optimized
Flow Cytometry : User Optimized
Western Blot : 1:1000-1:3000
ImmunoPrecipitation: User Optimized
Epitope Tag Type
RFP
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG2a
Label
Concentration
1 mg/ml by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
DsRed
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RFP Antibody Description

Background
Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS). RFP Proteins are useful markers for imagining protein localization, monitoring physiological processes, and detecting transgenic expression. Rockland's anti-RFP antibody can be used to detect native RFP and RFP variants.
Synonyms
RFP Antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
Immunogen
Anti-RFP monoclonal antibody is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Immunogen Type
Recombinant Protein
Storage Condition
Store anti-RFP at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-RFP antibodies is designed to detect Red Fluorescent Protein and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
Purity/Specifity
Anti-RFP Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Gross LA, Baird GS, Hoffman RC, Baldridge KK, Tsien RY: The structure of the chromophore within DsRed, a red fluorescent protein from coral. Proc. Natl. Acad. Sci. USA 2000, 97: 11990-11995.
Specific Reference
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Product Type
GFP and Epitopes;
Catalog Number
000-001-379

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC, IF
Catalog Number
600-401-379

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC
Catalog Number
600-403-379

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry