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Kits  >  Sepharose™ agarose Conjugates
 

TrueBlot® Anti-Goat Ig IP Beads

Rabbit Polyclonal
Code

00-8844-25
Size

2.5 mL
Price

$179.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Shipping & Handling Fees Apply.

Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: Anti-Goat immunoglobulin Gamma, Agarose-conjugated IgG, Rb-a-Gt IgG, Rabbit-anti-Goat IgG, TrueBlot, TrueBlot for immunoprecipitation, IP beads for TrueBlot.

TrueBlot® Anti-Goat Ig IP Beads Properties

Description
TrueBlot® Anti-Goat Ig IP Beads - 00-8844-25
Host
Rabbit
Target Species
Goat
Known Cross Reactivity
Goat
Clonality
Polyclonal
Application
Western Blot : Use with Goat TrueBlot® (cat # 18-8814-33)
ImmunoPrecipitation: TrueBlot anti-Goat Ig IP Beads (binds 1 mg Ig/ml beads) have been reported for use in IP
Product Type
Component
Kit Type
Immunoprecipitation Kit
Physical State
Suspension of agarose beads
Shipping condition
Wet Ice
Format
IgG
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.09% (w/v) Sodium Azide
Stabilizer
None
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Lot#  

TrueBlot® Anti-Goat Ig IP Beads Description

Background
TrueBlot® anti-Goat Ig IP Beads are a suspension of activated agarose beads coupled with rabbit anti-goat IgG. It is suitable for precipitation of goat IgGs used as the primary antibodies in immunoprecipitation assays. The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Storage Condition
Store vial at 4° C prior to opening. DO NOT FREEZE.
Application Note
Upon initial use of this product, we recommend that the vial be inverted several times to get the beads into suspension. We recommend to use a large bore pipet to pipet up the liquid for use. For storage of the opened vial, we recommend that the vial cap be sealed with parafilm to help prevent evaporation of the buffer. Procedure: Preparation of Immunoprecipitated Sample for SDS-PAGE: 1. Preclear cell lysate: Add 50 µl of anti-goat IgG beads and 500 µl of cell lysate sample to an eppendorf tube and incubate on ice for 30 minutes. Spin at 10,000xg for 3 minutes and transfer the supernatant to a new eppendorf tube. 2. Immunoprecipitation: Add 5 µg of primary antibody to the eppendorf tube containing the precleared lysate. Incubate on ice for 1 hour. Add 50 µl of Anti-Goat IgG Beads. Incubate for 1 hour on a rocking platform. Spin the eppendorf tube at 10000xg for 1 minute. Remove supernatant completely and wash the (pelleted) beads 3 times with 500 µl of Lysis Buffer. 3. Prepare sample for SDS-PAGE: After the last wash, aspirate supernatant, and add 100 µl Laemmli Buffer (with 50 mM DTT or 2% ß-mercaptoethanol, final) or NuPAGE LDS Sample Buffer (Invitrogen Cat#NP0007) containing NuPAGE Sample Reducing Agent (dithiothreitol; Invitrogen Cat#NP0004) to bead pellet. Vortex and heat to 90-100°C for 10 minutes. Spin at 10000xg for 3 minutes, collect supernatant, and load onto the gel. Avoid loading anti-goat Ig beads. Note: The supernatant can be stored at -20°C for future use. After thawing, add fresh NuPAGE Sample Reducing Agent (dithiothreitol) and heat as above. Centrifuge the sample at 10000xg for 1 minute in a microcentrifuge to pellet any anti-goat Ig beads and immediately transfer an aliquot of the supernatant to gel wells.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
WB
Reacts With
Goat
Catalog Number
18-8814-31

Product Type
Secondary Antibodies;
Applications
WB
Reacts With
Goat
Catalog Number
18-8814-33

Product Type
Secondary Antibodies;
Applications
WB
Reacts With
Sheep
Catalog Number
18-8815-31

Product Type
Secondary Antibodies;
Applications
WB
Reacts With
Sheep
Catalog Number
18-8815-33

Product Label

 

Description
Agarose beads for TrueBlot® IP/WB
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry