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Kits  >  Sepharose™ agarose Conjugates
 

TrueBlot® Anti-Rabbit Ig IP Beads

Goat Polyclonal
Code

00-8800-25
Size

2.5 mL
Price

$159.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
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Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: Anti-Rabbit Immunoglobulin Gamma, Agarose-conjugated IgG, Gt-a-Rb IgG, Goat-anti-Rabbit IgG, TrueBlot for immunoprecipitation, IP beads for TrueBlot.

TrueBlot® Anti-Rabbit Ig IP Beads Properties

Description
TrueBlot® Anti-Rabbit Ig IP Beads - 00-8800-25
Host
Goat
Target Species
Rabbit
Known Cross Reactivity
Rabbit
Clonality
Polyclonal
Application
Western Blot : Use with Rabbit TrueBlot® (cat # 18-8816-33)
ImmunoPrecipitation: TrueBlot anti-Rabbit Ig IP Beads (binds 2.5 mg Ig/ml beads) have been reported for use in IP
Species of Origin
Goat
Product Type
Component
Kit Type
Immunoprecipitation Kit
Physical State
Suspension of agarose beads
Shipping condition
Wet Ice
Format
IgG
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.09% (w/v) Sodium Azide
Stabilizer
None
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TrueBlot® Anti-Rabbit Ig IP Beads Description

Background
TrueBlot® anti-Rabbit Ig IP Beads are a suspension of activated agarose beads coupled with goat anti-rabbit IgG. It is suitable for precipitation of rabbit IgGs used as the primary antibodies in immunoprecipitation assays. The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Storage Condition
Store vial at 4° C prior to opening. DO NOT FREEZE.
Application Note
Upon initial use of this product, we recommend that the vial be inverted several times to get the beads into suspension. We recommend to use a large bore pipet to pipet up the liquid for use. For storage of the opened vial, we recommend that the vial cap be sealed with parafilm to help prevent evaporation of the buffer. Procedure: Preparation of Immunoprecipitated Sample for SDS-PAGE: 1. Preclear cell lysate: Add 50 µl of Anti-Rabbit IgG Beads and 500µl of cell lysate sample to a microcentrifuge tube and incubate on ice for 30 minutes. Spin at 10,000xg for 3 minutes and transfer thesupernatant to a new microcentrifuge tube. 2. Immunoprecipitation: Add 5 µg of primary antibody to the microcentrifuge tube containing the precleared lysate. Incubate on ice for 1 hour. Add 50 µl of Anti-Rabbit IgG Beads. Incubate for 1 hour on a rocking platform. Spin the microcentrifuge tube at 10000xg for 1 minute Remove supernatant completely and wash the (pelleted) beads 3 times with 500 µl of Lysis Buffer (50mM Tris HCl pH 8.0; 150mM NaCl;1% NP-40). 3. Prepare sample for SDS-PAGE: After the last wash, aspirate supernatant, and add 100 µl Laemmli Buffer (with 50 mM DTT or 2%ß-mercaptoethanol, final) to bead pellet. Vortex and heat to 90-100°C for 10 minutes. Spin at 10000xg for 3 minutes, collect supernatant, and load onto the gel. Avoid loading Anti-Rabbit Ig Beads. Note: The supernatant can be stored at -20°C for future use. After thawing, add fresh dithiothreitol and heat as above. Centrifuge the sample at 10000xg for 1 minute in a microcentrifuge tube to pellet any Anti-Rabbit Ig Beads and immediately transfer an aliquot of the supernatant to gel wells.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
WB
Reacts With
Rabbit
Catalog Number
18-8816-31

Product Type
Secondary Antibodies;
Applications
WB
Reacts With
Rabbit
Catalog Number
18-8816-33

Product Type
Kits;
Applications
WB, IP
Reacts With
Rabbit
Catalog Number
88-1688-31

Product Type
Kits;
Applications
WB, IP
Reacts With
Rabbit
Catalog Number
88-8886-31

Product Label

 

Description
Agarose beads for TrueBlot® IP/WB
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry