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Secondary Antibodies  >  Whole IgG Affinity Purified Secondary Antibodies
 

Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Phycoerythrin Conjugated

Rabbit Polyclonal
Code

610-408-C46
Size

1 mL
Price

$215.00
Availability

Ships within 2 weeks.
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Indirect ELISA of Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b, and 3) antibody.  
Antigen:  purified mouse IgG heavy and light chains.  
Coating amount:  0.1 µg per well.  
Primary antibody:  Rabbit Anti-Mouse IgG (Gamma 1, 2a, 2b, and 3) HRP conjugated Antibody.
Dilution series:  2-fold. 
Substrate:  TMB (p/n TMBE-0100).
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: rabbit anti mouse IgG Antibody, Rb-a-Mouse IgG Phycoerythrin conjugated, Rabbit IgG Phycoerythrin conjugated Secondary Antibody, Mouse IgG Phycoerythrin conjugated

Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Phycoerythrin Conjugated Properties

Description
Anti-MOUSE IgG (gamma 1, 2a, 2b and 3 chain) (RABBIT) Antibody Phycoerythrin Conjugated - 610-408-C46
Host
Rabbit
Specificity
IgG (gamma 1, 2a, 2b and 3 chain)
Target Species
Mouse
Known Cross Reactivity
Mouse
Clonality
Polyclonal
Application
IF Microscopy : 1:100 - 1:250
FlowCytometry : 1:100 - 1:250
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Concentration
0.5 mg/mL by absorbance = 82.0 at 565 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Lot#  

Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Phycoerythrin Conjugated Description

Background
Anti-Mouse IgG subclass pan reactive Secondary Antibody Phycoerythrin Conjugated is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays like western blotting.
Immunogen
Anti-Mouse IgG subclass pan reactive Secondary Antibody was pproduced by repeated immunization with highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
Application Note
Anti-Mouse IgG subclass pan reactive Secondary Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
Purity/Specifity
Anti-Mouse IgG subclass pan reactive Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Type
Supporting Reagents;
Applications
IHC
Catalog Number
MB-071-0100

Product Label

 

Description
R-Phycoerythrin (RPE)
Color
Red
Conjugation Reference
Modified from the method of Carlsson J, Drevin H, and Axen R. Protein thiolation and reversible protein-protein conjugation. Biochem J 173, 723-737 (1978).
Molecular Weight
240000
Excitation Wavelength
488
Conjugation Chemistry
SATA/MBS