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Secondary Antibodies  >  Whole IgG Affinity Purified Secondary Antibodies
 

Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Peroxidase Conjugated

Rabbit Polyclonal
Code

610-403-C46
Size

1 mg
Price

$179.00
Availability

Ships next business day.
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Indirect ELISA of Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b, and 3) antibody.  
Antigen:  purified mouse IgG heavy and light chains.  
Coating amount:  0.1 µg per well.  
Primary antibody:  Rabbit Anti-Mouse IgG (Gamma 1, 2a, 2b, and 3) HRP conjugated Antibody.
Dilution series:  2-fold. 
Substrate:  TMB (p/n TMBE-0100).
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Rabbit anti mouse IgG Antibody, Rb-a-Mouse IgG Peroxidase conjugated, Rabbit IgG peroxidase conjugated Secondary Antibody, Mouse IgG Peroxidase conjugated

Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Peroxidase Conjugated Properties

Description
Anti-MOUSE IgG (gamma 1, 2a, 2b and 3 chain) (RABBIT) Antibody Peroxidase Conjugated - 610-403-C46
Host
Rabbit
Specificity
IgG (gamma 1, 2a, 2b and 3 chain)
Target Species
Mouse
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2000 - 1:10000
Immunochemistry: 1:1000 - 1:5000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Concentration
1..0 mg/ml by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
500 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Gentamicin Sulfate
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Lot#  

Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Peroxidase Conjugated Description

Background
This Anti-Mouse IgG subclass pan reactive Secondary Antibody is is designed for Western Blotting, ELISA and Immunohistochemistry. HRP conjugated secondary antibodies can also be used for a variety of other applications such as Assay Development.
Immunogen
Highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.
Purity/Specifity
Anti-Mouse IgG subclass pan reactive Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Type
Supporting Reagents;
Applications
IHC
Catalog Number
MB-071-0100

Product Label

 

Description
Peroxidase (Horseradish)
Color
Substrate dependent
Conjugation Reference
Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Molecular Weight
40000
Excitation Wavelength
Conjugation Chemistry
Periodate