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Oxphos™ Cell Survival Assay Kit

NCI Collaboration JCYIA Product
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Oxphos™ Cell Survival Assay Kit Properties

Oxphos™ Cell Survival Assay Kit - KLD-002
Product Type
Product Type
Kit Type
Cell Survival Kit
Physical State
Shipping Condition
Wet Ice
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Oxphos™ Cell Survival Assay Kit Description

OxPhos™ is a medium based metabolic probe that can measure glutathione recycling capacity/glutathione function and glucose dependent antioxidant capacity of cells in tissue culture and whole blood. Oxphos™ is based on the ability of mammalian cells to rapidly and efficiently convert hydroxyethyl disulfide (HEDS) into mercaptoethanol (ME) through a bioreduction mechanism. Bioconversion of HEDS to ME relies on the activity of the oxidative pentose phosphate cycle (OPPC). OxPhos™ measures ME in the extracellular medium without the need for cellular lysis and extraction methods. Cell media is used in the assay, avoiding the need to lyse cells and thereby saving time and cost while preserving the ability to perform other cellular tests in the same culture system. OxPhos™ may have multiple applications in aging, oxidative stress, antioxidant screening, chemotherapy response and toxicology.
Glutathione recycling, glutathione function, and glucose dependent antioxidant capacity assay, cell viability, cell survival cellular viability, cellular survival, antioxidant assay
Storage Condition
Store kit at 2-8° C prior to opening. See kit insert for complete instructions.
Application Note
Oxphos™ gives a linear response for mammalian cells (0; 100,000; 200,000; 400,000; 600,000; and 800,000) plated in 1mL growth medium in a six well plate with up to 15% fetal bovine serum and measured 20 hours after plating. It also gives a linear response for human blood (0, 10, 20, 40, 60, 80, 100 µL) suspended in a total volume of 200 µL saline in a microfuge tube and incubated with 10 µL of reagent 6 for 2hrs. Oxphos™ contains enough reagents for 100 assays using a a 6-well plate.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
Jie Li, Donglan Zhang, Pearl A. Jefferson, Kathleen M. Ward, and Iraimoudi S. Ayene. (2014) A bioactive probe for glutathione-dependent antioxidant capacity in breast cancer patients: Implications in measuring biological effects of arsenic compounds. Journal of Pharmacological and Toxicological Methods. Vol:69, Issue 1, Jan–Feb: Pg: 39–48.
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