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Primary Antibodies  >  Kinase and Phosphatase Antibodies
 

CaMKII Antibody

Mouse Monoclonal 6G9 IgG1
Code

200-301-F00
Size

100 µg
Price

$309.00
Availability

Ships within 2 weeks.
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Immunofluorescence Microscopy of mouse anti-CaMKII antibody. Tissue: Hippocampal neurons. Fixation: N/A. Primary antibody: anti-CaMKII  for 1h at RT. Seondary antibody: Fluorescein mouse secondary antibody at 1:10,000 for 45 min at RT.  Localization: Cytoplasm.  Staining: CaMKII as precipiated ed signal.
Western Blot of mouse anti-CaMKII anitbody.  Lane 1: Rat membrane. Load: 10ug.  Primary antibody: CaMKII at 1:1000 overnight at 4°C.  Secondary antibody: Goat anti-mouse IgG HRP at 1:40,000 for 45 min at RT.  Blocked: 5% Blotto overnight at 4°C. Predicated/observed size:  54kDa, ~54 kDa for CaMKII.
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Synonyms: CamK2, CamK2A, CamK2B, CamK2D, CamK2G, CAMKA, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha

CaMKII Antibody Properties

Description
Anti-CaMKII (MOUSE) Monoclonal Antibody - 200-301-F00
Host
Mouse
Target Species
Rat
Known Cross Reactivity
Mouse, Rat, Bovine
Clonality
Monoclonal 6G9 IgG1
Application
ELISA : 1:200
IF Microscopy : User Optimized
Western Blot : 0.1µg/ml (1:10,000)
Immunohistochemistry: User Optimized
ImmunoPrecipitation: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG1
Label
Concentration
1mg/mL by UV absorbance at 280 nm
Buffer
See application note.
Preservative
0.09% (w/v) Sodium Azide
Stabilizer
50% (v/v) Glycerol
GeneName
Camk2a
Download CofA
Lot#  

CaMKII Antibody Description

Background
CaMKII is an important member of calcium/calmodulin- activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site. The binding of Ca2+ auto inhibitory effect and activates the kinase . This kinase activation results in autophosphorylation at threonine 286. The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation. Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities.
Immunogen
CaMKII Antibody was produced in mice by repeated immunizations with a partially purified rat CaMKII.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-CaMKII Antibody is suitable for IF, IP, WB, IHC and RIA/ELISA. Antibody is provided in PBS pH 7.4. Expect a band approximately ~50-60kDa protein, corresponding to the molecular mass of CaMKII a-subunit on SDS page immunoblots. Recognizes both phosphorylated and non-phosphorylated forms. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-CaMKII Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with CaMKII from mouse, rat, and bovine based on 100% homology with the immunizing sequence. Cross-reactivity with CaMKII from other sources has not been determined. Cell Signaling research.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Hughes K. et al. (2001) J. Biol. Chem. 276: 36008–36013. Barria A. et al. (1997) Science 276: 2042–2045. Bennet M.K. and Kennedy M.B. (1987) Proc. Natl. Acad. Sci. U.S.A. 84: 1794-1798. Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808. Nghiem P., Saati S. M., Martens C. L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479. Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102. Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292. Means A.R. (2000) Mol. Endocrinol. 14: 4–12. Makhinson M. et al. (1999) J. Neurosci. 19: 2500–2510. Strack S. and Colbran R.J. (1998) J. Biol. Chem. 273: 20689-20692. Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244. Shen K. and Meyer Y. (1999) Science 284: 162-167.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, dog
Catalog Number
600-401-945

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, monkey, dog, mouse, rat
Catalog Number
600-401-967

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-401-A77

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry