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Secondary Antibodies  >  ATTO Conjugated Antibodies
 

Mouse IgG2b Antibody ATTO 550 Conjugated Pre-adsorbed

Goat Polyclonal
Code

610-154-042
Size

500 µg
Price

$279.00
Availability

Ships within 2 weeks.
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ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal.   Examples shown are: 
A.  Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody.
B.  Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red).
C.  HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue).
D.  Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.
Images provided courtesy of Dr. Jörg Reichwein,  ATTO-TEC GmbH
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: IgG2b antibody, mouse IgG2b, anti-subclass secondary antibody, AT550, ATTO 550, ATTO-TEC 550

Mouse IgG2b Antibody ATTO 550 Conjugated Pre-adsorbed Properties

Description
Anti-MOUSE IgG2b (Gamma 2b chain) (GOAT) Antibody ATTO 550 Conjugated (Min Cross Reactivity to Bv, Hu, and Rb Serum Proteins) - 610-154-042
Host
Goat
Specificity
IgG2b
Target Species
Mouse
Known Cross Reactivity
Mouse
Clonality
Polyclonal
Application
FLISA : >1:20,000
IF Microscopy : >1:5,000
FlowCytometry : 1:500 - 1:2,500
Western Blot : >1:10,000
Immunochemistry: >1:5,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG2b
Label
FP Value
2.5 moles ATTO 550 per mole of IgG2b
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
500 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Mouse IgG2b Antibody ATTO 550 Conjugated Pre-adsorbed Description

Background
ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Immunogen
Mouse IgG2b heavy chain
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Mouse IgG2b secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2b antibody based assays. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
Purity/Specifity
Anti-MOUSE IgG2b (Gamma 2b chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2b. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Type
Supporting Reagents;
Applications
IHC
Catalog Number
MB-071-0100

Product Label

 

Description
ATTO 550
Color
Orange
Conjugation Reference
Molecular Weight
791
Excitation Wavelength
554
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester