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Kits  >  Loading Control Antibodies
 

Loading Control Antibody Sampler Kit

Code

KAS-001
Size

1 Kit
Price

$315.00
Availability

Ships within 2 weeks.
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Western Blot of Rabbit Anti-Beta Actin Antibody.  
Lane 1:  molecular weight.  
Lane 2:  human embryonic kidney 293.  
Lane 3:  human lung carcinoma A549.  
Lane 4:  mouse brain.  
Load:  35 µg per lane.
Primary antibody:  Beta Actin antibody at 1:1,500 for overnight at 4°C.
Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  ~42 kDa corresponding to beta Actin (arrowhead). 
Other band(s): none.
Western Blot of Rabbit Anti-HDAC-1 Antibody.  
Lane 1:  293 whole cell lysate.  
Lane 2:  none.  
Load:  35 µg per lane.
Primary antibody:  HDAC-1 antibody at 1:3,500 for overnight at 4°C.
Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  ~65 kDa corresponding to human HDAC1. 
Other band(s): none.
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Synonyms:

Loading Control Antibody Sampler Kit Properties

Description
LOADING CONTROL ANTIBODY SAMPLER KIT -KAS-001
Application
ELISA : 1:5,000 - 1:25,000
Western Blot : 1:500 - 1:5,000
Other Dilution: User Optimized
Product Type
Kit
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Label
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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Lot#  

Loading Control Antibody Sampler Kit Description

Background
The Loading Control Antibody Sampler Kit contains antibodies to a variety of housekeeping proteins. Loading controls are essential for the interpretation of western blot data. Loading control antibodies allow the verification of equal protein loading between samples. Ideal loading controls are expressed constitutively and at high levels with low variability between cell lines and experimental conditions. Also, the loading control should be at a different molecular weight than the protein of interest this allows the bands to be visually distinguishable. The Loading Control Antibody Sampler kit contains enough primary and secondary antibodies to perform ten western blots per loading control antibody.
Immunogen
VDAC, GAPDH, alpha-Tubulin, HDAC-1, beta-Actin
Storage Condition
Store vials at -20° C or below prior to opening. The primary antibodies contain a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
Each kit contains one unit of the following products: Anti-VDAC/Porin (RABBIT) Antibody (25 µL) Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (RABBIT) Antibody (25 µL) Anti-alpha-Tubulin (RABBIT) Antibody (25 µL) Anti-HDAC-1 (RABBIT) Antibody (25 µL) Anti-beta Actin (RABBIT) Antibody (25 µL) Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (2 mg)
Purity/Specifity
All loading control antibodies included in this kit - VDAC/Porin Antibody, GAPDH Antibody, alpha-Tubulin Antibody, HDAC-1 Antibody and beta-Actin Antibody are affinity purified against there respective targets.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Kits;
Catalog Number
KBB-001

Product Type
Cell Lysates;
Applications
WB
Catalog Number
W09-000-361

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry