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Primary Antibodies  >  AKT Signaling Antibodies
 

AKT2 Antibody

Rat Monoclonal 16G11.E8 IgG2a kappa
Code

200-501-E71
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Western Blot of Rat anti-AKT2 antibody.  
Lane 1:  GST Tagged recombinant AKT1.  
Lane 2:  GST Tagged recombinant AKT2.  
Lane 3:  GST Tagged recombinant AKT3.
Load:  25 ng per lane.
Primary antibody:  AKT2 antibody at 1:1,000 for overnight at 4°C.
Secondary antibody:  Peroxidase rat secondary antibody at 1:40,000 for 30 min at RT.
Block:  MB-070 for 30 min at RT.
Predicted/Observed size: 78 kDa for AKT2. 
Other band(s): none.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: AKT 2 antibody, AKT-2, PKB antibody, PKB beta antibody, PKBBETA antibody, PRKBB antibody, Protein kinase Akt 2 antibody, Protein kinase B beta antibody, RAC-beta serine/threonine-protein kinase, RAC-PK-beta

AKT2 Antibody Properties

Description
Anti-AKT2 (RAT) Monoclonal Antibody - 200-501-E71
Host
Rat
Target Species
Human
Known Cross Reactivity
Human
Clonality
Monoclonal 16G11.E8 IgG2a kappa
Application
ELISA : 1:2,000 - 1:10,000
FlowCytometry : User Optimized
Western Blot : 1:500- 1:2000
Immunochemistry: 20 µg/mL
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG2a
Label
Concentration
1mg/ml by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
AKT2
Related Links
Download CofA
Lot#  

AKT2 Antibody Description

Background
AKT2 Antibody detects AKT2 which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT2 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Immunogen
Anti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-AKT2 Antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT2 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
Purity/Specifity
Anti-AKT2 antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT2 was purified from concentrated tissue culture supernate by Protein G chromatography. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 88% identity to mouse and 90% identity with rat, therefore, cross reactivity is expected.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Altomare, D. A., Kozak, C. A., Sonoda, G. and Testa, J. R. (1996) Chromosome mapping of the mouse Akt2 gene and Akt2 pseudogene. Cytogenet. Cell Genet. 74: 248-251. PubMed ID : 8976376 Cheng, J. Q. wt al. (1992) AKT2, a putative oncogene encoding a member of a subfamily of protein-serine/threonine kinases, is amplified in human ovarian carcinomas. Proc. Nat. Acad. Sci. 89: 9267-9271. PubMed ID : 1409633 Cheng, J. Q. et al. (1996) Amplification of AKT2 in human pancreatic cancer cells and inhibition of AKT2 expression and tumorigenicity by antisense RNA. Proc. Nat. Acad. Sci. 93: 3636-3641. PubMed ID : 8622988
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
human, mouse, rat, chimpanzee
Catalog Number
200-301-401

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-B19

Product Type
Primary Antibodies;
Applications
ELISA, WB, FC
Reacts With
human
Catalog Number
600-401-425

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry