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Avidin, Biotin, Streptavidin  >  ATTO Conjugated Antibodies
 

STREPTAVIDIN ATTO 425 Conjugated

Code

S000-51
Size

500 µg
Price

$159.00
Availability

Ships next business day.
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Atto Dye-Immunofluorescence microscopy

ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal.   Examples shown are: 
A.  Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody.
B.  Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red).
C.  HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue).
D.  Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.
Western Blot of Atto 425 Conjugated Streptavidin.
Lane 1:  Biotin conjugated Guinea Pig IgG.
Lane 2:  None.
Load:  50 ng per lane.
Primary antibody:  None.
Secondary antibody:  Atto 425 Conjugated Streptavidin at 1:1,1000 for 60 min at RT.
Block:  MB-070 for 30 min at RT.
Predicted/Observed size:  28 & 55 kDa, 28 & 55 kDa for Guinea Pig IgG.
Other band(s):  None.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: AT425, ATTO 425, ATTO-TEC 425, STREPTAVIDIN ATTO 425 Conjugated

STREPTAVIDIN ATTO 425 Conjugated Properties

Description
STREPTAVIDIN ATTO 425 Conjugated - S000-51
Application
FLISA : >1:20,000
IF Microscopy : >1:5,000
Western Blot : >1:10,000
Reagent Type
Streptavidin
Physical State
Lyophilized
Shipping condition
Ambient
Label
FP Value
3.5 moles ATTO 425 per mole of
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
500 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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STREPTAVIDIN ATTO 425 Conjugated Description

Background
STREPTAVIDIN ATTO 425 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
Purity/Specifity
STREPTAVIDIN ATTO 425 was prepared from chromatographically purified Streptavidin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Streptavidin. No reaction was observed against anti-Avidin.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Type
Avidin, Biotin, Streptavidin;
Applications
IP
Catalog Number
PA50-00-0002

Product Label

 

Description
ATTO 425
Color
Blue
Conjugation Reference
Molecular Weight
498
Excitation Wavelength
436
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester