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Supporting Reagents  >  Blocking Solutions and BSA
 

Revitablot™ Western Blot Stripping Buffer

Code

MB-085-0500
Size

500 mL
Price

$105.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
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Western Blot comparison of Revitablot™ Western Blot Stripping Buffer.  
Lane 1:  70 µg HeLa whole cell lysates.  
Lane 2:  10 µg HeLa whole cell lysates.  
Blot A first probed with: 
Primary antibody: Rabbit Anti-STAT5 (p/n 600-401-A44) at a dilution of 1:1,000 overnight at 4°C.
Secondary antibody: HRP Goat anti-rabbit (p/n 611-103-122) at 1:10,000 dilution using FemtoMax Super Sensitive Chemiluminescent HRP Substrate (p/n FEMTOMAX-110).
Membranes were then incubated with Revitablot and a competitor's product for 20 minutes at room temperature, followed by re-blocking with 5% BLOTTO for 2 hours at room temperature. 
Blot B (Blot A reprobed) with:
Primary antibody: Rabbit anti-tubulin (p/n 600-401-880) at a dilution of 1:1,000 overnight at 4°C.
Secondary antibody: HRP Goat anti-rabbit (p/n 611-103-122) at 1:10,000 dilution using FemtoMax Super Sensitive Chemiluminescent HRP Substrate (p/n FEMTOMAX-110).
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Stripping solution, Western Blot stripping buffer, western blot reprobing buffer, bound antibody removal buffer

Revitablot™ Western Blot Stripping Buffer Properties

Description
Revitablot™ Western Blot Stripping Buffer - MB-085-0500
Application
Western Blot : Use undiluted.
Product Type
Buffer
Product Type
Solution
Substrate/Enzyme Type
Blocking Reagent
Product Type
Blocking Reagent
Physical State
Liquid
Shipping condition
Ambient
Label
Buffer
See application note.
Preservative
None
Stabilizer
None
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Lot#  

Revitablot™ Western Blot Stripping Buffer Description

Background
Revitablot™ Western Blot Stripping Buffer is a uniquely formulated, ready-to-use reagent specifically designed for western blotting. Revitablot™ Western Blot Stripping Buffer is faster and more efficient at stripping of primary and secondary antibodies, including HRP and fluorescent labeled. Blots can be stripped multiple times for the repeated use of membranes. This solution can be used at room temperature. Only incubation of 5-20 minutes needed to strip the membrane. Revitablot™ Western Blot Stripping Buffer is gentle and effective on both nitrocellulose and PVDF western blot membranes.
Storage Condition
Store container at room temperature (18° to 26° C) prior to opening.
Application Note
Revitablot™ Western Blot Stripping Buffer is ready-to-use as a working 1X solution and requires no further dilution.
Purity/Specifity
Revitablot™ Western Blot Stripping Buffer contains solutions in a proprietary combination to enhance the removal of bound antibodies from western blot membranes for repeated use. The proprietary formulation of the solution ensures high stripping efficency with low backgrounds.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Controls;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat. chicken, bovine
Catalog Number
200-301-880

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat. chicken, bovine
Catalog Number
600-401-880

Product Type
Controls;
Applications
ELISA, WB, IHC, IF
Reacts With
Mouse, Human, Rat, Rabbit, Chicken, Bovine, Swine
Catalog Number
600-401-A33

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry