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Primary Antibodies  >  Cell Cycle Protein Antibodies
 

BIN1 Antibody

Mouse Monoclonal 99F IgG
Code

200-301-E61
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Phone: 800.656.7625
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Immunofluorescence Microscopy of Mouse Anti-BIN1 Antibody.
Cells: C2C12 cells during growth or differentiation.
Fixation:  0.5% PFA.
Antigen retrieval:  not required.
Primary antibody: BIN-1 (Exon 10 specific, 99F) monoclonal antibody.
Secondary antibody:  mouse secondary antibody at 1:10,000 for 45 min at RT.
Localization:  BIN1 is nuclear and cytoplasmic.
Staining:  BIN 1 as green fluorescent signal.
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Shipping:
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Synonyms: AMPHL, Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, BIN 1, BIN-1, BIN1 antibody, anti-BIN1 antibody

BIN1 Antibody Properties

Description
Anti-BIN1 (MOUSE) Monoclonal Antibody - 200-301-E61
Host
Mouse
Target Species
Human
Known Cross Reactivity
mouse, human
Clonality
Monoclonal 99F IgG
Application
ELISA : 1:5000-1:50000
Western Blot : 1:500-1:1500
Immunohistochemistry: 1:100-1:500
ImmunoPrecipitation: 10-100 µL
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
BIN1
Related Links
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Lot#  

BIN1 Antibody Description

Background
Bin1 is a conserved member of the BAR family of genes that have been implicated in diverse cellular processes including endocytosis, actin organization, programmed cell death, stress responses, and transcriptional control. The first mammalian BAR protein to be discovered, Amphiphysin I (AmphI), was identified in an immunoscreen for proteins associated with the plasma membranes of synaptic neurons, functions in the control of clathrin-dependent synaptic vesicle endocytosis. The mammalian Bin1 gene was first identified in a two hybrid screen for polypeptides that bind to the N-terminal Myc box 1 (MB1) portion of the c-Myc oncoprotein. Bin1 is similar to AmphI in overall structure, with an N-terminal BAR domain and a C-terminal SH3 domain. However, the Bin1 gene is more complex than the AmphI gene, encoding at least seven different splice variants that differ widely in subcellular localization, tissue distribution, and ascribed functions. Alternate splicing of the Bin1 gene results in ten transcript variants encoding different isoform. Bin1 is expressed ubiquitously in mammalian cells. Certain splice variants of Bin1 are expressed in the neurons, muscle cells or tumor cells and play a role in cancer suppression. Studies in muscle cells suggest that Bin1 expression, structure, and localization are tightly regulated during muscle differentiation and suggested that Bin1 plays a functional role in the differentiation process. Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive; also known as autosomal recessive myotubular myopathy.
Immunogen
Anti-BIN1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with BIN1 polypeptide followed by hybridoma development.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-BIN1 antibody has been tested for use in ELISA, Western Blot, IP, and IF. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-BIN1 was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. BIN1 antibody is specific for human BIN1 protein. A BLAST analysis was used to suggest cross-reactivity with BIN1 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with BIN1 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Wechsler-Reya, R., D. et al. (1998) Mol Cell Biol 18:566-575. Nicot A. S. et al. (2007) Nat Genet. 39:1134-1139. Wechsler-Reya, R., D. et al. (1997) J. Biol Chem 272:31453-31458. Prendergast G. C. et al. (2009) Biochem. Biophys. Acta. 1795:25-36. Tsutsui, K. et al. (1997) Biochem. Biophys. Res. Commun. 236:178-183. Ramjaun, A. R. et al. (1997) J. Biol. Chem. 272:16700-16706. Butler, M. H. J. et al. (1997) Cell Biol. 137:1355-1367. Sakamuro, D. et al. (1996) Nat. Genet. 14:69-77. Lichte, B. R. et al. (1992) EMBO J. 11:2521-2530.
Specific Reference
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Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC
Catalog Number
600-401-381

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry