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Primary Antibodies  >  Cancer Research Antibodies
 

IDO1 Antibody

Mouse Monoclonal 10.1 IgG3
Code

200-301-E57
Size

100 µg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Western Blot of Mouse Anti-IDO1 Antibody.
Lane 1:  untreated HeLa cells.
Lane 2:  IFN-r treated HeLa cells.
Load:  35 µg per lane.
Primary antibody: IDO 1 Antibody at 1:1000 for overnight at 4°C.
Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  41-42 kDa, 41-42 kDa for IDO-1.
Other band(s): none.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Ido, Indo, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase, Ido1, Ido-1

IDO1 Antibody Properties

Description
Anti-IDO1 (MOUSE) Monoclonal Antibody - 200-301-E57
Host
Mouse
Target Species
Human
Known Cross Reactivity
human, mouse
Clonality
Monoclonal 10.1 IgG3
Application
ELISA : 1:5000-1:50000
IF Microscopy : 1:50-1:100
Western Blot : 1:500-1:1500
Immunochemistry: 1:100-1:500
ImmunoPrecipitation: 10-100 µL
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG3
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
Ido1
Related Links
Download CofA
Lot#  

IDO1 Antibody Description

Background
Indoleamine 2, 3-dioxygenase1 (IDO1) is a 41-42 kD intracellular enzyme that catabolizes tryptophan into kynurenine. IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO1 effects on immune suppression are due to decreased tryptophan availability and the generation of tryptophan metabolites, resulting in negative effects on T lymphocytes, including proliferation, function and survival. IDO1 may be involved in the suppression of the immune response to tumors, and blocking the IDO1 pathway may be a potential target for immuno and cancer therapy. IDO1 is expressed in a wide variety of tissues and can be upregulated by interferon gamma and other inflammatory cytokines.
Immunogen
Anti-IDO1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with fragment of recombinant human and mouse IDO1 protein followed by hybridoma development.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-IDO1 antibody has been tested for use in ELISA, Western Blot, IP, IHC, and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-IDO1 was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human and mouse IDO1 protein. A BLAST analysis was used to suggest cross-reactivity with IDO1 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with IDO1 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Mellor AL., et al. (2004) Nat Rev Immunol 4:762-774. Godin-Ethier J., et al. (2011) Clin Cancer Res 17:6985-6991. Mellor AL., et al. (2002) J Immunol 168:3771-3776. Frumento G., et al. (2002) J Exp Med 196:459-468. Muller AJ., et al. (2005) Nat Med 11:312-319. Lob S., et al. (2009) Nat Rev Cancer 9:445-452. Uyttenhove C., et al. (2003) Nat Med 9:1269-1274. Daubener w., et al. (1999) Adv Exp Med Biol 467:517-524.
Specific Reference
Batista CAE., Juhász C., Muzik O., Kupsky WJ., Barger G., Chugani HT., Mittal S., Sood S., Chakraborty PK., Chugani DC., (2009) Imaging Correlates of Differential Expression of Indoleamine 2,3-Dioxygenase in Human Brain Tumors. Mol Imaging Biol 11:460Y466. DOI: 10.1007/s11307-009-0225-0. Meyer KC., Klatte JE., Dinh H.V., Harries M.J., Reithmayer K., Meyer W., Sinclair R., Paus R. (2008) Evidence that the bulge region is a site of relative immune privilege in human hair follicles. British Journal of Dermatology 159, pp1077–1085. Lingqiao Zhu, Fang Ji, Yuan Wang, Yi Zhang, Qiang Liu, Jingwu Z. Zhang, Kouji Matsushima, Qi Cao and Yanyun Zhang. (2006) Synovial Autoreactive T Cells in Rheumatoid Arthritis Resist IDO-Mediated Inhibition. J. Immunol.177;8226-8233.
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-1302

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry