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Primary Antibodies  >  Stem Cell Antibodies
 

Sprouty-2 Antibody

Rabbit Polyclonal
Code

600-401-E68
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Immunohistochemistry of Rabbit anti-Sprouty2 antibody.
Tissue:  human kidney clear cell tumor. 
Fixation:  formalin fixed, paraffin embedded. 
Antigen retrieval: yes.
Primary antibody:  Sprouty2 antibody at 1:250. 
Secondary antibody:  alexafluor 488 secondary at 1:500 overnight at 4°C. 
Localization:  nucleus and cytoplasm. 
Staining: green = sprouty2, Blue = dapi, red= vhl.
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Synonyms: hSPRY 2, hSPRY2, MGC23039, Sprouty homolog 2 (Drosophila), Sprouty homolog 2, Sprouty2, Spry 2, sprouty-2, sprouty 2

Sprouty-2 Antibody Properties

Description
Anti-Sprouty-2 (RABBIT) Antibody - 600-401-E68
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : User Optimized
IF Microscopy : User Optimized
Western Blot : 1:500-1:5,000
Immunochemistry: 10 µg/mL
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.04 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
Sprouty 2
Related Links
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Lot#  

Sprouty-2 Antibody Description

Background
Sprouty was originally identified as an inhibitor of Drosophila epidermal growth factor (EGF) and fibroblast growth factor (FGF) receptor signaling during tracheal development. Four isoforms of mammalian sprouty are known (Spry1-Spry4). The role of the mammalian analogs has not been clearly elucidated although it is believed that human Sprouty-2 (hSpry2) may be an inhibitor of cellular migration and proliferation. Spry2 and Spry4 show considerable sequence homology between human and mouse at the C-terminus of the protein. Significant sequence divergence occurs at the N-terminus. Spry2 appears to be abundant in brain, lung and heart tissue, with lesser amounts found in kidney and skeletal muscle.
Immunogen
Anti-Sprouty-2 affinity purified antibody was purified from monospecific rabbit antiserum prepared via repeated immunizations with peptide corresponding to an internal region of human sprouty-2.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Sprouty-2 antibody is suitable for ELISA, Immunofluorescence, Immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-Sprouty-2 was affinity purified from monospecific antiserum by immunoaffinity chromatography. Sprouty-2 antibody reacts with human Sprouty 2 protein. A BLAST analysis was used to suggest reactivity with Sprouty 2 from human, monkey, and orangutan based on a 100% homology with the immunizing sequence. Cross-reactivity with Sprouty2 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Yigzaw, Y. et al. (2001) The Carboxy Terminus of Sprouty is Important for Modulation of Cellular Migration and Proliferation. J. Biol. Chem. 276, 22742-22747. Reich, A. et al. (1999) Sprouty is a general inhibitor of receptor tyrosine kinase signaling. Development 126 (18) 4139-4147. Hacohen, N. et al. (1998) Sprouty encodes a novel antagonist of FGF signaling that patterns apical branching of the Drosophila airways. Cell 92: 253-263
Specific Reference
Zhang, C., Chaturvedi, D. Jaggar, L., Magnuson, D., Lee, J. and Patel, T.B. (2005) Regulation of Vascular Smooth Muscle Cell Proliferation and Migration by Human Sprouty 2. Arterioscler Thromb Vasc Biol 25: 533 - 538.
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
600-401-241

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, chimpanzee
Catalog Number
600-401-697

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry