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Primary Antibodies  >  DNA Damage and Repair Antibodies
 

p53 phospho S392 Antibody

Rabbit Polyclonal
Code

600-401-E04
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Western Blot of Rabbit anti-p53 pS392 antibody.  
Lane 1:  rat brain nuclear fraction lysate (control).  
Lane 2:  ?-Ptase.  
Load:  10 µg per lane.
Primary antibody:  p53 pS392 antibody at 1:1,000 for overnight at 4°C.
Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  53 kDa for p53 pS392. 
Other band(s): none.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

p53 phospho S392 Antibody Properties

Description
Anti-p53 pS392 (Rabbit) Antibody - 600-401-E04
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, rat
Clonality
Polyclonal
Application
Western Blot : 1:1000
Other Dilution: User Optimized
Physical State
Liquid
Shipping condition
Dry Ice
Format
IgG
Label
Buffer
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
GeneName
TP53
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Lot#  

p53 phospho S392 Antibody Description

Background
p53 pS392 Antibody detects phophorylated p53. p53 has a well established role in blocking the proliferative action of damaged cells and acting in essence as an anticancer agent. It has been called the guardian of the genome. Phosphorylation of Ser392 in p53 is associated with formation of human tumors. In addition, p53 has been linked to effects of aging and oxidative stress. An increase in p53 has also been linked to deficits in LTP and learning and memory. Anti-p53 pS392 Antibody is ideal for investigators involved in Transcription Factor, Cell Cycle, Apoptosis, and Cancer research.
Immunogen
Anti-p53 pS392 Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser392.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Application Note
Anti-p53 pS392 Antibody is suitable for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 53 kDa in size corresponding to p53 protein phosphorylated at Ser392 in the appropriate cell lysate or extract.
Purity/Specifity
Anti-p53 pS392 antibody is directed against p53. The anibody was affinity purified from monospecific antiserum by immunoaffinity purification and is specific for the phosphorylated protein. The immunolabeling is completely eliminated by ?-phosphatase treatment. Reactivity is expected the following species based on 100% sequence homology: human and rat. Cross reactivity with p53 from other species has not been determined
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Jiang YH, Armstrong D, Albrecht U, Atkins CM, Noebels JL, Eichele G, Sweatt JD and Beaudet AL (1998). Mutation of the angelman ubiquitin ligase in mice causes increased cytoplasmic p53 and deficits of contextual learning and long-term potentiation. Neuron 21:799-811. Kim YY, Park BJ, Kim DJ, Kim WH, Kim S, Oh KS, Lim JY, Kim J, Park C and Park SI (2004). Modification of serine 392 is a critical event in the regulation of p53 nuclear export and stability. FEBS Lett 572:92-98. Lane DP (1992). p53, guardian of the genome. Nature (London) 358:15-16. Pise-Masison CA, Radonovich M, Sakaguchi K, Appella E and Brady JN (1998). Phosphorylation of p53: a novel pathway for p53 inactivation in human T- cell lymphotropic virus type 1-transformed cells. J Virol 72:6348-6355. Saito S, Yamaguchi H, Higashimoto Y, Chao C, Xu Y, Fornace AJ, Jr., Appella E and Anderson CW (2003). Phosphorylation site interdependence of human p53 post-translational modifications in response to stress. J Biol Chem 278:37536-37544. Sharpless NE and DePinho RA (2002). p53: Good cop/bad cop. Cell 110:9-12. Yin Y, Tainsky MA, Bischoff FZ, Strong LC and Wahl GM (1992). Wild-type p53 restores cell cycle control and inhibits gene amplification in cells with mutant p53 alleles. Cell 70:937-948.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, chicken
Catalog Number
100-401-401

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP, ChIP
Reacts With
human
Catalog Number
200-301-174

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat
Catalog Number
200-301-400

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry