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Nuclear Pore Complex Antibody

Mouse Monoclonal 39C7 IgG1
NCI Collaboration
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Immunofluorescence Microscopy of Mouse anti-Nuclear Pore Complex antibody.  
Tissue:  HeLa cells.  
Fixation:  0.5% PFA  
Antigen retrieval:  not required.
Primary antibody:  Nuclear Pore Complex antibody  at 1:50-1:100  for 1 h at RT.
Secondary antibody:  Fluorescein mouse secondary antibody at 1:10,000 for 45 min at RT.
Localization:  Nuclear Pore Complex spans the double membrane of the nuclear membrane.
Staining:  Nuclear Pore Complex as green fluorescent signal with chicken anti-vimentin as red fluorescent signal.
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Number:
900-301-D98
Size:
100 µL
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships within 2 weeks.

Nuclear Pore Complex Antibody Properties

Description
Anti-Nuclear Pore Complex (Mouse) Antibody - 900-301-D98
Host
Mouse
Target Species
Yeast
Known Cross Reactivity
yeast, human, mouse, rat
Clonality
Monoclonal 39C7 IgG1
Application
IF Microscopy : 1:50-100
Other Dilution: User optmimized
Physical State
Liquid
Shipping Condition
Dry Ice
Format
IgG1
Label
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
GeneName
Nuclear stain of multiple gene products including Nup62, Nup133
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Nuclear Pore Complex Antibody Description

Background
Nuclear Pore Complex Antibodydetects Nuclear Pore Complex proteins. The nuclear pore complex (NPC) is a very large structure made up of at least 50 different proteins that span the double membrane of the nuclear envelope functioning as a gateway for macromolecular traffic between the cytoplasm and the nucleus. Discrete nuclear pore complex proteins or nucleoporins such as NUP98, NUP180 and p62 have been implicated in autoimmune disease and cancer. Patients with primary biliary cirrhosis (PBC) frequently produce autoantibodies against p62 and NUP180 while NUP98 translocations have been found in patients with acute myelogenous leukemia (AML). Anti-Nuclear Pore Complex Antibody is ideal for investigators involved in Cancer and Immunology Research.
Synonyms
NPC
Immunogen
Anti-Nuclear Pore Complex Antibody (Monoclonal) was produced by repeated immunizations with yeast nuclear preparations.
Immunogen Type
Native Protein
Storage Condition
Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Application Note
Anti-Nuclear Pore Complex Antibody is suitable for use in IF. Specific conditions for reactivity should be optimized by the end user
Purity/Specifity
Anti-Nuclear Pore Complex antibody is directed against Nuclear Pore Complex proteins. The antibody is a total fraction of IgG from concentrated clarified cell culture supernate. This antibody is directed against yeast NPC protein. Reactivity is expected from human, mouse and rat.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Nakielny S and Dreyfuss G (1999). Transport of proteins and RNAs in and out of the nucleus. Cell 99(7):677-690. Wilken N, Kossner U, Senecal JL, Scheer U and Dabauvalle MC (1993). Nup180, a novel nuclear pore complex protein localizing to the cytoplasmic ring and associated fibrils. J Cell Biol 123(6 pt 1):1345-54. Nesher G, Margalit R and Ashkenazi YJ (2001). Anti-nuclear envelope antibodies: Clinical associations. Semin Arthritis Rheum 30(5):313-20. Jaju RJ, Fidler C, Haas OA, Strickson AJ, Watkins F, Clark K, Cross NC, Cheng JF, Aplan PD, Kearney L, Boultwood J and Wainscoat JS (2001). A novel gene, NSD1, is fused to NUP98 in the t(5;11)(q35;p15.5) in de novo childhood acute myeloid leukemia. Blood 98(4):1264-7.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, chicken
Catalog Number
100-401-401

Product Type
Controls;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat. chicken, bovine
Catalog Number
200-301-880

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
mouse
Catalog Number
610-103-121

Product Type
Molecular Biology Reagents;
Catalog Number
MB-009-1000

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry