Western Blot of Anti-GluR1 (Mouse) Antibody - 200-301-D61

Datasheet

GluR1 Antibody

Name: GluR1 Antibody
Brand: Rockland Immunochemicals
Price: 485.0 USD
Availability: OutOfStock

Mouse Monoclonal RH95 IgG2a

1 References

Item No. 200-301-D61

Size: 100 µL

Applications: WB, IF

Reactivity: Mouse, Rat

Host Species: Mouse

$485.00 /Per Item

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Background

GluR1 Monoclonal Antibody detects GluR1. The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain. GluR1 monoclonal antibody is ideal for investigators involved in Neuroscience.

Expand Background

Specifications for GluR1 Antibody

Product Details

Description: Anti-GluR1 (MOUSE) Monoclonal Antibody - 200-301-D61

Synonyms: Glutamate receptor 1, GluR1, AMPA-selective glutamate receptor 1, GluR-A, GluR-K1, Glutamate receptor ionotropic, AMPA 1

Host Species: Mouse

Clonality: Monoclonal

Format: IgG2a

Target Details

Gene Name: Gria1 - View All Gria1 Products

Reactivity: Mouse, Rat

Immunogen Type: Conjugated Peptide

Immunogen: Anti-GluR1 Monoclonal Antibody was produced by repeated immunizations with GluR1- Synthetic peptide corresponding to amino acid residues from the N-terminal region.

Purity/Specificity: Anti-GluR1 Monoclonal Antibody is directed against rat GluR1 protein. The antibody was Protein G purified from cell culture supernatant. Reactivity is expected against mouse and human. Cross reactivity with GluR1 from other species has not been determined.

Database Links:

UniProtKB - P19490

GeneID - 50592

UniProtKB - P19490.2

Application Details

Tested Applications: WB

Suggested Applications: IF - View References

Application Note: Anti-GluR1 (Mouse) antibody has been tested by Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 100 kDa in size corresponding to GluR1 proteins in Western Blot in the appropriate cell lysate or extract.

Formulation

Physical State: Liquid

Concentration: Titrated value sufficient to run approximately 10 mini blots.

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (1)

Dominguez-Sala E et al. (2022). Properties of the epileptiform activity in the cingulate cortex of a mouse model of LIS1 dysfunction. Brain Struct Funct.

Applications

IF, Confocal Microscopy; WB, IB, PCA

PubMed

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.