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Primary Antibodies  >  Neuroscience Antibodies
 

GABA(A) Receptor delta Antibody

Rabbit Polyclonal
Code

600-401-C80
Size

100 µL
Price

$309.00
Availability

Ships within 2 weeks.
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Western Blot of Rabbit anti-GABAA Receptor d N-Terminus Antibody.  
Lane 1:  mouse cerebellar lysates from wild type.  
Lane 2:  mouse cerebellar lysates from d-knockout (d-K/O).  
Load:  10 µg per lane.
Primary antibody:  GABAA-R antibody at 1:400 for overnight at 4°C.
Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:   ~52kDa/~52kDa for d-subunit of the GABAA-R. 
Other band(s):  none.
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Synonyms: Gamma-aminobutyric acid receptor subunit delta, GABA(A) receptor subunit delta, Gabrd

GABA(A) Receptor delta Antibody Properties

Description
Anti-GABA(A) Receptor delta (Rabbit) Antibody - 600-401-C80
Host
Rabbit
Target Species
Rat
Known Cross Reactivity
rat, mouse
Clonality
Polyclonal
Application
ELISA : 1:5000-1:25,000
Western Blot : 1:1,000
Immunohistochemistry: 1:250
ImmunoPrecipitation: 10µl per 50µg lysate
Physical State
Liquid
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
Titrated value sufficient to run approximately 10 mini blots. by UV absorbance at 280 nm
Buffer
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Preservative
None
Stabilizer
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
GeneName
GABRD
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GABA(A) Receptor delta Antibody Description

Background
Anti-GABA(A) Receptor delta Antibody detects GABA(A) Receptor delta. Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six as, four ßs and four ?s, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for a- and ß-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, co-expression of a ?-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different a-subunits of the receptor. More recently there have been a number of studies demonstrating that the d-subunit of the receptor may affect subunit assembly and may also confer differential sensitivity to neurosteroids and to ethanol. GABA(A) receptor delta antibody is ideal for investigators involved in Neuroscience.
Immunogen
Anti-GABAA Receptor delta Antibody was produced by repeated immunizations with recombinant fusion protein from the N-terminal region of the delta subunit.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Application Note
Anti-GABA(A) Receptor delta (Rabbit) antibody (600-401-C80) is tested for use in Western Blotting, and is suitable for Western blotting and other immunoassays. Specific conditions should be optimized by the end user. Expect a band of approximately 52 kDa in size corresponding to the delta subunit of the GABA(A) proteins.
Purity/Specifity
Anti-GABA(A) Receptor delta Antibody is directed against rat GABA(A) Receptor delta. The antibody was affinity purified from monospecific antiserum by immunoaffinity purification. This antibody is directed against rat GABAA receptor delta protein. Reactivity is expected from mouse. Cross reactivity with GABA(A) Receptor delta from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Korpi ER, Mihalek RM, Sinkkonen ST, Hauer B, Hevers W, Homanics GE, Sieghart W and Luddens H (2002). Altered receptor subtypes in the forebrain of GABAA receptor d-subunit-deficient mice: recruitment of ?2-subunits. Neurosci 109:733-743. McKernan RM, et al. (2000). Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor a1-subtype. Nature Neurosci 3:587-592. Mehta AK and Ticku MK (1998). Prevalence of the GABAA receptor assemblies containing a1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration. Mol Brain Res 67:194-199. Ogris W, Pöltl A, Hauer B, Ernst M, Oberto A, Wulff P, Höger H, Wisden W and Sieghart W (2004). Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor ?2-subunit. Biochem Pharmacol 68:1621-1629. Olsen RW and Tobin AJ (1990). Molecular biology of GABAA receptors. FASEB 4:1469-1480. Pöltl A, Hauer B, Fuchs K, Tretter V and Sieghart W (2003). Subunit composition and quantitative importance of GABAA receptor subtypes in the cerebellum of mouse and rat. J Neurochem 87:1444-1455. Wallner M, Hanchar HJ and Olsen RW (2003). From The Cover: Ethanol enhances a4ß3d and a6ß3d ?-aminobutyric acid type A receptors at low concentrations known to affect humans. Proc Natl Acad Sci (USA) 100:15218-15223. Whiting PJ, Bonnert TP, McKernan RM, Farrar S, Le Bourdellès B, Heavens RP, Smith DW, Hewson L, Rigby MR, Sirinathsinghji DJS, Thompson SA and Wafford KA (1999). Molecular and functional diversity of the expanding GABAA receptor gene family. Ann NY Acad Sci 868:645-653. Wohlfarth KM, Bianchi MT and Macdonald RL (2002). Enhanced Neurosteroid Potentiation of Ternary GABAA Receptors Containing the d-Subunit. J Neurosci 22:1541-1549.
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-106-122

Product Type
Avidin, Biotin, Streptavidin;
Applications
ELISA, WB, IHC
Catalog Number
S000-03

Product Type
Supporting Reagents;
Applications
ELISA
Catalog Number
TMBE-1000

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry