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Primary Antibodies  >  Cytokines & Growth Factor Antibodies
 

Anti-Human IL-17A (MOUSE) Biotin Conjugated Antibody

Mouse Monoclonal 4H1524.1 IgG2b
Code

209-306-B32
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western Blot of Mouse Anti-IL-17A antibody  
Lane 1:  human full length recombinant IL-17A protein
Lane 2:  mouse full length recombinant IL-17A protein
Lane 3:  rat full length recombinant IL-17A protein
Load: 20 ng/lane 
Primary antibody: Anti-IL-17A antibody at 1ug/mL for overnight at 4°C
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Interleukin-17, IL-17, IL-17A, Interleukin-17A, Cytotoxic T-Lymphocyte-associated Antigen 8, CTLA8.

Anti-Human IL-17A (MOUSE) Biotin Conjugated Antibody Properties

Description
Anti-Human IL-17A (MOUSE) Biotin Conjugated Monoclonal Antibody - 209-306-B32
Host
Mouse
Target Species
Human
Known Cross Reactivity
human
Clonality
Monoclonal 4H1524.1 IgG2b
Application
ELISA : 1:10,000-1:50,000
FlowCytometry : User Optimized
Western Blot : 1µg/mL
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG2b
Label
FP Value
10-20 moles Biotin per mole of IgG2b
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
GeneName
IL17A
Download CofA
Lot#  

Anti-Human IL-17A (MOUSE) Biotin Conjugated Antibody Description

Background
IL17-A (also known as Interleukin-17) is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. IL17-A is the founding member of a group of cytokines called the IL17 family. IL17-A was originally identified as a transcript from a rodent T-cell hybridoma. To elicit its functions, IL17 binds to a type I cell surface receptor called IL17R of which there are at least three variants IL17RA, IL17RB, and IL17RC. Anti-IL-17A antibody is ideal for investigators involved in Immunology research.
Immunogen
Anti-IL-17A (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17A produced in E.coli followed by hybridoma development.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration.   Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
This purified antibody has been tested for use in FLOW and Western Blot. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-17A protein. A BLAST analysis was used to suggest cross-reactivity with IL-17A from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-17A from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Paust,H.J., Turner,J.E., Steinmetz,O.M., Peters,A., Heymann,F., Holscher,C., Wolf,G., Kurts,C., Mittrucker,H.W., Stahl,R.A. and Panzer,U. (2009) The IL-23/Th17 axis contributes to renal injury in experimental glomerulonephritis. J. Am. Soc. Nephrol. 20 (5), 969-979. Yin,Y., Wen,S., Li,G. and Wang,D. (2009) Hypoxia enhances stimulating effect of amyloid beta peptide (25-35) for interleukin 17 and T helper lymphocyte subtype 17 upregulation in cultured peripheral blood mononuclear cells. Microbiol. Immunol. 53 (5), 281-286. Shin,J.H., Shin,D.W. and Noh,M. (2009) Interleukin-17A inhibits adipocyte differentiation in human mesenchymal stem cells and regulates pro-inflammatory responses in adipocytes. Biochem. Pharmacol. 77 (12), 1835-1844.
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-B32

Product Type
Primary Antibodies;
Applications
ELISA, WB, FC
Reacts With
human
Catalog Number
209-301-B32

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
209-401-B32

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Human
Catalog Number
209-403-B32

Product Label

 

Description
Biotin
Color
n/a
Conjugation Reference
Modified from Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
Molecular Weight
455
Excitation Wavelength
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester