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Primary Antibodies  >  Cytokines & Growth Factor Antibodies
 

IL-17F Antibody

Mouse Monoclonal 4H1629.1 IgG1
Code

209-301-B31
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Immunohistochemistry of Mouse Anti-IL-17F antibody  
Tissue:  human colon tissue 
Fixation: formalin-fixed, paraffin-embedded 
Primary antibody: isotype control (top left) , Mouse Anti-IL-17F antibody (bottom left, right) at 5 ug/ml
Western Blot of Mouse Anti-IL-17F antibody  
Lane 1:  human full length recombinant IL-17F protein
Lane 2:  mouse full length recombinant IL-17F protein
Lane 3:  rat full length recombinant IL-17F protein
Load: 20 ng/lane 
Primary antibody: Anti-IL-17F antibody at 0.5ug/mL for overnight at 4°C
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Interleukin-17F, Cytokine ML-1, Interleukin-24

IL-17F Antibody Properties

Description
Anti-Human IL-17F (MOUSE) Monoclonal Antibody - 209-301-B31
Host
Mouse
Target Species
Human
Known Cross Reactivity
human
Clonality
Monoclonal 4H1629.1 IgG1
Application
ELISA : 1:10,000-1:50,000
Western Blot : 0.5µg/mL
Immunochemistry: 5µg/mL
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG1
Label
Concentration
1.0mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
IL17F
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Lot#  

IL-17F Antibody Description

Background
Anti-L-17F recognizes IL-17F (also known as Cytokine ML-1 or Interleukin-24). IL-17F is produced and secreted by CD8+ T cells, NK cells, NKT cells and LTi cells. The main functions of IL-17F are neutrophil recruitment and immunity to extracellular pathogen. More importantly, IL-17F drives inflammation and auto-immunity. IL-17A and IL-17F are by far the best characterized cytokines of the IL-17 cytokine family. IL-17F dimerizes in a parallel fashion similar to nerve growth factor and other neutrophins. Its dimerization is critical to fulfill its activity. When secreted by activated T cells, IL-17F can stimulate the production of other cytokines such as IL-6, IL-8 granulocyte colony-stimulating factor and, can stimulate cartilage matrix turnover. Defects in IL17F are the cause of familial candidiasis type 6 (CANDF6). CANDF6 is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. Anti-IL-17E cytokine antibody is ideal for investigators involved in Immunology, Signal Transduction research, Cancer and Inflammatory pathologies.
Immunogen
Anti-IL-17F (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17F produced in E.coli followed by hybridoma development.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Human IL-17F antibody has been tested for use in IHC and Western Blot. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-Human IL-17F (MOUSE) Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-17F protein. A BLAST analysis was used to suggest cross-reactivity with IL-17F from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-17F from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Basso AS, Cheroutre H, Mucida D. (2009) More stories on Th17 cells. Cell Research 19:399–411. doi: 10.1038/cr.2009.26; published online 3 March 2009. Conti HR, Shen F,Nayyar N, Stocum E, Sun JN, Lindemann MJ, Ho AW, Hoda Hai J, Yu JJ, Won Jung J, Filler SG, Masso-Welch P, Edgerton M, Gaffen SL. (2009) Th17 cells and IL-17 receptor signaling are essential for mucosal host defense against oral candidiasis. J Exp Med. February 16; 206(2): 299–311. doi: 10.1084/jem.20081463. Hymowitz S.G. (2001) IL-17s adopt a cysteine knot fold: structure and activity of a novel cytokine, IL-17F, and implications for receptor binding. et al., EMBO J. Xu S., Cao X. (2010) Interleukin-17 and its expanding biological functions. Cellular & Molecular Immunology. Korn T. (2009) IL-17 and Th17 Cells. Korn T. et al. Annual Review of Immunology.
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-B31

Product Type
Primary Antibodies;
Applications
ELISA, WB, FC
Reacts With
Human
Catalog Number
209-301-310

Product Type
Primary Antibodies;
Applications
ELISA, WB, FC
Reacts With
human
Catalog Number
209-301-B32

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
209-301-B61

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry