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Supporting Reagents  >  Blocking Solutions and BSA
 

GOAT SERUM

Code

B304
Size

10 mL
Price

$105.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Goat Serum blocking reagent or NGS is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding.  This blocking agent is recommended for use in immunoassays where the primary antibody was produced in goat, as a source of non-specific serum protein or on tissue for immunohistochemical applications.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: blocking goat serum, blocking grade, 10% NGS

GOAT SERUM Properties

Description
NORMAL GOAT SERUM (NGS) - B304
Application
ELISA : Yes
FLISA : Yes
IF Microscopy : Yes
FlowCytometry : Yes
Western Blot : Yes
Immunochemistry: Yes
ImmunoPrecipitation: Yes
Blocker Type
Serum
Substrate/Enzyme Type
Blocking Reagent
Product Type
Blocking Reagent
Physical State
Lyophilized
Shipping condition
Ambient
Label
Concentration
92 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
10 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide and 0.01% (w/v) Gentamicin Sulfate
Stabilizer
None
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Lot#  

GOAT SERUM Description

Background
Goat Serum blocking reagent or NGS is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding.
Storage Condition
Store normal goat serum at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Goat Serum blocking reagent or NGS can be used as a blocking agent to treat plastic surfaces, membrane or tissue after they have been sensitized with primary antibody or antigen. It provides an alternative to bovine serum albumin (BSA) and non-fat dry milk. It is effective in reducing nonspecific binding of proteins to reaction surfaces, thereby maximizing signal-to-noise ratio. This blocking agent is recommended for use in immunoassays where the primary antibody was produced in goat, as a source of non-specific serum protein or on tissue for immunohistochemical applications.
Purity/Specifity
Goat Serum blocking reagent or NGS was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation.  Assay by immunoelectrophoresis resulted in a multiple precipitin arcs against anti-Goat Serum. Normal Goat Serum was obtained from non-immunized healthy goats.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry