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Supporting Reagents > Enzyme Substrates

TMB Membrane Peroxidase Substrate. Ready-To-Use

Code

TMBM-100

Size

100 mL

Price

$90.00

Availability

Ships next business day.

Order Offline:

Phone: 800.656.7625

E-mail: orders@rockland-inc.com

Fax: 610.367.7825

Bulk Orders:

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Rockland TMB membrane substrate, chemiluminescent substrate and secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein (011-0102, 250ng starting total load) co incubated with Rockland HRP conjugated Goat anti Rabbit IgG (611-103-122) and Dylight 649 conjugated goat anti Rabbit (611-143-122) 1:20K in MB-070. Blot was dried and imaged (A) on Biorad Versa Doc (30 sec, DyLight649),
(B) LiCor Odyssey Reader (700 nm),
(C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec),
(D) Incubated with TMB substrate (TMBM-100) for 5 minutes and scanned, and
(E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager,

Shipping & Handling Fees Apply.

Shipping:

$40.00 to United States

Handling:

$35.00

Synonyms: 3,3’,5,5’-Tetramethylbenzidine, TMB-M, TMBM

Datasheet
References
Customer Reviews
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TMB Membrane Peroxidase Substrate. Ready-To-Use Properties

Description

TMB MEMBRANE PEROXIDASE SUBSTRATE. READY-TO-USE - TMBM-100

Application

Western Blot : 1x
Other Dilution: 1x

Substrate/Enzyme Type

Substrate

Product Type

Substrate

Physical State

Liquid - clear

Shipping condition

Ambient

Label

Unconjugated

Concentration

N/A

Stabilizer

Proprietary buffer with enhancer

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Lot#

TMB Membrane Peroxidase Substrate. Ready-To-Use Description

Background

TMB Membrane Peroxidase Substrate (3, 3', 5, 5' - Tetramethylbenzidine) is a chromogenic substrate used to visualize antibody reactivity in ELISA experiments. In the presence of peroxidases, TMB can act as an electron donor for the conversion of peroxides to water, changing the color of solution to a blue color equivalent to the degree of reactivity. This reaction of TMB can be halted with acid to change the TMB to yellow. TMB Membrane Peroxidase Substrate (3, 3', 5, 5' - Tetramethylbenzidine) is ideal for investigators in Immunology, Cancer, and Microbiology research.

Storage Condition

Store container at 4° C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required.

Application Note

TMB Membrane Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB Membrane Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB Membrane Peroxidase Substrate comes ready to use.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Martin, Mufson and Mesulam. (1984). "The light side of horseradish peroxidase histochemistry". J Histochem Cytochem 32 (7): 793.

Specific Reference

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