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Avidin, Biotin, Streptavidin  >  Avidin-Biotin Reagents
 

STREPTAVIDIN PEROXIDASE Conjugated

Code

S000-03
Size

1 mg
Price

$149.00
Availability

Ships next business day.
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Western Blot of Peroxidase Conjugated Streptavidin.  
Lane 1:  Human IL-7.  
Lane 2:  none.  
Load:  50 ng per lane.
Primary antibody:  Human IL-7 Biotin Conjugated antibody at 1:1,000 for overnight at 4°C.
Secondary antibody:  Peroxidase Conjugated Streptavidin at 1:40,000 for 30 min at RT.
Block:  5% BLOTTO 30 min at RT.
Predicted/Observed size:  17 kDa, 17 kDa for Human IL-7. 
Other band(s): none.
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Shipping:
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Handling:
$20.00
Synonyms: HRP, Horseradish peroxidase, SA, S avidin

STREPTAVIDIN PEROXIDASE Conjugated Properties

Description
STREPTAVIDIN PEROXIDASE Conjugated - S000-03
Application
ELISA : 1:20,000 - 1:200,000
Western Blot : 1:10,000 - 1:40,000
Immunochemistry: 1:1,000 - 1:5,000
Other Dilution: User Optimized
Reagent Type
Streptavidin
Physical State
Lyophilized
Shipping condition
Ambient
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Related Links
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STREPTAVIDIN PEROXIDASE Conjugated Description

Background
Streptavidin is a a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditions that would typically cause dissociation (such as organic solvents, denaturants, detergents, and extreme temperatures or pH). Streptavidin's afinity for biotin can be employed in a variety of experimental uses, from purifications to standards, to means of detection or pull down experiments. Horse Raddish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Streptavidin Peroxidase conjugated is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Streptavidin Peroxidase is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in Immunohistochemistry, Western Blotting, and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).
Purity/Specifity
Streptavidin Peroxidase was prepared from chromatographically purified streptavidin.  Streptavidin Peroxidase was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin.  No reaction was observed against anti-Avidin.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Custom Products;
Applications
ELISA, WB, IP
Catalog Number
CUST09


Product Type
Molecular Biology Reagents;
Applications
ELISA
Catalog Number
MB-064-1000

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Peroxidase (Horseradish)
Color
Substrate dependent
Conjugation Reference
Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Molecular Weight
40000
Excitation Wavelength
Conjugation Chemistry
Periodate