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Avidin, Biotin, Streptavidin  >  Avidin-Biotin Reagents
 

STREPTAVIDIN

NCI Collaboration
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SDS-Page of Streptavidin.
Lane 1: Molecular weight markers.
Lane 2: Streptavidin.
Load: 1.0 ug per lane.
Predicted/Observed size: The molecular weight of streptavidin is 55,000 daltons. The protein is composed of 4 essentially identical polypeptide chains (homotetramer). This product is chromatographically pure Streptavidin and shows predominantly a single 13.800 dalton band by SDS-PAGE.
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Number:
S000-01
Size:
5 mg
Price:
$205.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

STREPTAVIDIN Properties

Description
STREPTAVIDIN - S000-01
Application
ELISA : 1:20,000-1:200,000
ChIP : User Optimized
GelShift : User Optimized
Western Blot : 1:2000-1:20,000
Reagent Type
Streptavidin
Physical State
Lyophilized
Shipping Condition
Ambient
Label
Concentration
0.9 mg/mg streptavidin/lyophilizate (balance is sodium chloride).
Specific Activity
16.2 U/mg by biotin titration method
Buffer
0.15 M Sodium Chloride
Reconstitution Volume
5.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
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STREPTAVIDIN Description

Background
Streptavidin is a a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditions that would typically cause dissociation (such as organic solvents, denaturants, detergents, and extreme temperatures or pH). Streptavidin's afinity for biotin can be employed in a variety of experimental uses, from purifications to standards, to means of detection or pull down experiments.
Synonyms
SA, S avidin, streptococcus avidin
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Streptavidin is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Streptavidin is suitable for use as antigen, as a control or standard in assays, and most other immunological methods as well as enzyme conjugates and complexes; Southern blots and other methodologies related to DNA and RNA analysis; Western blots; and purification of proteins or other antigens with biotinylated antibodies or lectins by use of immobilized streptavidin.
Purity/Specifity
Streptavidin is chromatographically pure and shows predominantly a single 53,600 dalton band by SDS-PAGE.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Agaraña, C. E., Kuntz, I. D., Birken, S., Axel, R. and C. R. Cantor. Molecular cloning and nucleotide sequence of the streptavidin gene. Nucleic Acids Res. 14: 871-882 (1986).
Specific Reference
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Product Type
Avidin, Biotin, Streptavidin;
Applications
ELISA, WB, IHC
Catalog Number
B000-03

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Type
Avidin, Biotin, Streptavidin;
Applications
ELISA, WB, IHC
Catalog Number
S000-03

Product Type
Supporting Reagents;
Applications
ELISA
Catalog Number
TMBE-1000

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry