Protein G Biotin Conjugated
Assay results using Biotin Protein G
Assay using Biotin Protein G
Assay using Biotin Protein G
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Datasheet

Protein G Biotin Conjugated

4 References
PG00-06
500 µg
IF, FC
Streptococcus sp.
Protein G Protein G Fluorescein Conjugated Protein G Peroxidase Conjugated Protein G Alkaline Phosphatase Conjugated Protein G DyLight™ 488 Conjugated Protein G DyLight™ 549 Conjugated Protein G DyLight™ 649 Conjugated Protein G DyLight™ 680 Conjugated Protein G DyLight™ 800 Conjugated Protein G DyLight™ 405 Conjugated
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Background

Protein G is a surface protein of two groups of Streptococcal bacteria that has the ability to bind immunoglobulins. Similar to Protein A, but with slightly different specificity, Protein G is an important agent in the purification of proteins due to its ability to bind the Fc region. While native Protein G binds to albumin, recombinant Protein G is designed to contain only immunoglobulin binding domains to ensure the maximum specific IgG binding capacity. Protein G Biotin Conjugated is ideal for investigators involved in Signal Transduction, Molecular Biology, Cancer, and Cell Signaling.

Product Details

Protein G Biotin Conjugated - PG00-06
ProG, Streptococcus G protein, Protein G Biotin Conjugated
Streptococcus sp.
Biotin

Target Details

Protein G Biotin Conjugated was prepared from chromatographically pure recombinant Protein G.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Protein G. No reaction was observed against anti-Protein A.
UniProtKB - P19909

Application Details

FC, IF  - View References
Protein G Biotin Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype.

Formulation

Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
500 µL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
(). A viral genome packaging ring-ATPase is a flexibly coordinated pentamer. Nature Communications
Applications
IF, Confocal Microscopy
(). A Bead-Based Flow Cytometric Assay for Monitoring Yersinia pestis Exposure in Wildlife. J Clin Microbiol.
Applications
FC, FACS, FLOW
(). Designing a nine cysteine-less DNA packaging motor from bacteriophage T4 reveals new insights into ATPase structure and function. Virology
Applications
FC, FACS, FLOW
(). A promiscuous DNA packaging machine from bacteriophage T4. PloS Biology
Applications
IF, Confocal Microscopy

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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