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PROTEIN A - PA00-00
PROTEIN A - PA00-00
Avidin, Biotin, Streptavidin  >  Sepharose™ agarose Conjugates
 

SEPHAROSE™ PROTEIN A

Code

PA50-00-0002
Size

2 mL
Price

$199.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Sepharose™ Protein A is a suspension of beads conjugated to a fusion protein containing binding domains from protein A from Staphylococcus aureaus.
Ideal for Western Blotting where photo sensitive Conjugated Antibodies such as IRDye Conjugated Antibodies and DyLight Conjugated Antibodies are used. Rockland Immunochemicals features both large and small boxes to fit varying size and volume requirements.
Shipping & Handling Fees Apply.

Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: Sepharose Protein A

SEPHAROSE™ PROTEIN A Properties

Description
SEPHAROSE™ PROTEIN A - PA50-00-0002
Application
ImmunoPrecipitation: User Optimized
Other Dilution: User Optimized
Physical State
Suspension of agarose beads
Shipping condition
Wet Ice
PSC Format
Agarose Conjugate
Label
Concentration
6.0 mg/cc drained sepharose gel (~1 cc settled sepharose beads per 2 ml suspension)
Buffer
20% (v/v) Ethanol
Stabilizer
None
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SEPHAROSE™ PROTEIN A Description

Background
Sepharose™ Protein A is a suspension of beads conjugated to native protein A derived from Staphylococcus aureaus. When evenly dispersed approximately 2.0 cc of suspension is equal to 1.0 cc of settled beads of Sepharose™ 4 Fast Flow (highly cross linked, 4% agarose derivative). The bead size is approximately 45-165 µm when swollen. Approximately 6.0 mg of Protein A is bound per cc of drained beads. Each cc of drained beads will bind approximately 35 mg Human. The exclusion limit (Mr) of the beads is 3 x 10^7. The maximum linear flow rate is approximately 1300 cm/hr. This product shows long term stability within pH range of 3 to 9 and short term stability within pH range of 2 to 10.
Storage Condition
Store SEPHAROSE PROTEIN A at 4° C prior to opening. DO NOT FREEZE.
Application Note
Protein A resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.
Purity/Specifity
Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2, and rabbit. Immobilization of protein A creates an affinity resin that can be used to isolate IgG fractions from crude serum, ascites fluid or hybridoma supernatants/dilute cell cultures. High capacity and high flow rate fractionation is applicable to both laboratory and scale-up processes. Sepharose™ Protein A can be used for immunoprecipitation and purification of monoclonal antibodies.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Molecular Biology Reagents;
Catalog Number
MB-001

Product Type
Molecular Biology Reagents;
Catalog Number
MB-003

Product Type
Molecular Biology Reagents;
Catalog Number
MB-008

Product Type
Avidin, Biotin, Streptavidin;
Applications
ELISA, WB
Catalog Number
PA00-00

Product Label

 

Description
Sepharose™
Color
n/a
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry