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Avidin, Biotin, Streptavidin  >  Avidin-Biotin Reagents
 

Protein A Alkaline Phosphatase Conjugated

Code

PA00-05
Size

1 mg
Price

$175.00
Availability

Ships next business day.
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Synonyms: ProA, ALP, Alk Phos

Protein A Alkaline Phosphatase Conjugated Properties

Description
PROTEIN A ALKALINE PHOSPHATASE Conjugated - PA00-05
Application
ELISA : 1:8,000 - 1:32,000
Western Blot : 1:500 - 1:2,500
Immunohistochemistry: 1:200 - 1:1,000
Other Dilution: User Optimized
Reagent Type
Protein A
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Preservative
0.1% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Protein A Alkaline Phosphatase Conjugated Description

Background
Protein A is a surface protein (approximately 56kDa in size) originally discovered within the cell wall of Staphylococcus aureus. While important for bacterial survival, Protein A has beneficial uses in immunology for its high affinity binding to immunoglobulins (especially the IgG isotype). This high affinity property makes Protein A essential in the large scale purification of antibodies. Alkaline Phosphatase is an enzyme which removes phosphate groups from a variety of substate molecules. As the name implies, this enzyme functions best under basic pH. Alkaline Phosphatase can be utilized in molecular biology in DNA ligation experiments (keeping the DNA linear), radiolabeling preparations, and a detection mediator in ELISA experiments.
Storage Condition
Store vial at 4° C before opening. DO NOT FREEZE. Protein A Alkaline Phosphatase Conjugated is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Application Note
Protein A Alkaline Phosphatase Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader.
Purity/Specifity
Protein A Alkaline Phosphatase Conjugated was prepared from chromatographically pure Protein A.   Protein A Alkaline Phosphatase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Protein A.  No reaction was observed against anti-Protein G.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Supporting Reagents;
Applications
ELISA
Catalog Number
NPP-10

Product Type
Supporting Reagents;
Applications
ELISA
Catalog Number
NPP-B500

Product Type
Avidin, Biotin, Streptavidin;
Applications
ELISA, WB, IHC
Catalog Number
PA00-03-1000

Product Type
Avidin, Biotin, Streptavidin;
Applications
ELISA, WB, IHC
Catalog Number
PG00-05

Product Label

 

Description
Alkaline Phosphatase (Calf Intestine)
Color
Substrate dependent
Conjugation Reference
Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175, 1971.
Molecular Weight
140000
Excitation Wavelength
Conjugation Chemistry
Glutaraldehye coupling