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Avidin, Biotin, Streptavidin  >  Avidin-Biotin Reagents
 

Protein A

NCI Collaboration
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Number:
PA00-00
Size:
5 mg
Price:
$105.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

Protein A Properties

Description
PROTEIN A - PA00-00
Application
ELISA : User Optimized
Western Blot : User Optimized
Other Dilution: User Optimized
Reagent Type
Protein A
Physical State
Lyophilized
Shipping Condition
Ambient
Label
Concentration
N/A
Reconstitution Volume
5.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
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Protein A Description

Background
Protein A is a surface protein (approximately 56kDa in size) originally discovered within the cell wall of Staphylococcus aureus. While important for bacterial survival, Protein A has beneficial uses in immunology for its high affinity binding to immunoglobulins (especially the IgG isotype). This high affinity property makes Protein A essential in the large scale purification of antibodies.
Synonyms
ProA, Staphylococcus A protein
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Protein A is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Protein A is suitable for use as an antigen, as a control or standard in assays, for conjugation and for most other immunological methods. This recombinant Protein A contains only IgG binding domains, ensuring maximum specific IgG binding.
Purity/Specifity
Protein A is chromatographically pure and shows predominantly a single band by SDS-PAGE.  Greater than 95% of the A280 material binds to Human IgG.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Avidin, Biotin, Streptavidin;
Applications
ELISA, WB, IHC
Catalog Number
PA00-03

Product Type
Avidin, Biotin, Streptavidin;
Applications
ELISA, WB, IHC
Catalog Number
PA00-05

Product Type
Avidin, Biotin, Streptavidin;
Applications
ELISA, WB, IHC
Catalog Number
PA00-06

Product Type
Avidin, Biotin, Streptavidin;
Applications
ELISA, WB
Catalog Number
PG00-00

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry