The latest software is used to analyze your DNA or protein sequence to project the physical characteristics of the expressed protein, including extinction coefficient, isoelectric point, charge, hydrophobicity and secondary structure. BLAST is performed to compare your sequence to published sequences to determine common and species-specific regions to aid with the design of peptides. A complete restriction site analysis is performed to aid in vector construction. A search for silent mutations that result in the appearance or disappear-ance of restriction sites without changing the protein sequence is performed. Oligonucleotides for PCR amplification, sequencing primers, and hybridization probes are designed and analyzed for melting temperatures, dimer and hairpin loop formation.