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Molecular Biology Reagents  >  Molecular Biology Products
 

Deoxyribonuclease I

Code

MB-101-0100
Size

100 Units
Price

$69.00
Availability

Ships next business day.
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Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms:

Deoxyribonuclease I Properties

Description
Deoxyribonuclease I (Bovine Pancreas) RNAse and Protease Free Molecular Biology Grade - MB-101-0100
Application
Other Dilution: User Optimized
Product Type
Enzyme
Product Type
Reagent
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Label
Buffer
See application note.
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Lot#  

Deoxyribonuclease I Description

Background
Deoxyribonuclease I is an endonuclease which splits phosphodiester linkages yielding polynucleotides with a free 3’-OH and 5’-phosphate. Activated by bivalent metal ions and inhibited by chelators such as EDTA. Chromatographically purified to be free of ribonuclease and protease activity.
Storage Condition
Store vial at 4° C or at -20° C or colder prior to opening product.
Application Note
One unit will cause an increase in the absorbance at 260 nm of 0.001 per minute per ml at 25° C, pH 5.0 when acting on highly polymerized DNA according to the assay method of Kunitz (J.Gen.Physiol. 33, 349 and 363 (1950)). A solution in 1 mM Calcium Chloride and 50% (v/v) glycerol.
Purity/Specifity
Enzyme Concentration: 2290 units/ml or ~2.2 units/ul (100 units/vial) which approximately equals 1 mg/ml. % Ribonuclease: None detected % Protease: None detected
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry