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Supporting Reagents  >  Blocking Solutions and BSA
 

Blocking Buffer For Immunohistochemistry Serum And Azide Free

Code

MB-071-0100
Size

100 mL
Price

$99.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Immunohistochemistry Protein Blocker is the best first choice for blocking paraffin fixed or frozen sectioned tissues specimens for immunohistochemical staining for immunoenzymatic signal processing.  IHC Protein Blocker is suitable for manual or automated IHC staining systems.
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Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms:

Blocking Buffer For Immunohistochemistry Serum And Azide Free Properties

Description
Blocking Buffer for Immunohistochemistry - Serum and Azide Free - MB-071-0100
Application
Immunohistochemistry: Undiluted or 1:1
Blocker Type
Other
Substrate/Enzyme Type
Blocking Reagent
Product Type
Blocking Reagent
Physical State
Liquid (sterile filtered)
Shipping condition
Ambient
Label
Concentration
N/A
Buffer
See application note.
Preservative
Thimerosal is added as an antimicrobial agent.
Stabilizer
Proprietary
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Lot#  

Blocking Buffer For Immunohistochemistry Serum And Azide Free Description

Background
A detailed protocol for immunohistochemical staining is available at www.rockland-inc.com.
Storage Condition
Store container at 4° C before opening.  DO NOT FREEZE. 
Application Note
This immunohistochemistry protein blocker is specifically designed for immunohistochemical staining where detection occurs using an immunoenzymatic reaction with substrate, i.e. peroxidase (HEP) with diaminobenzidine (DAB). This product specifically inhibits non-specific staining of IHC detection reagents. It may be used on various specimens, including cultured cells and primary tissue to lower background staining. Optimum performance is achieved using this product undiluted.  However, Immunohistochemistry Protein Blocker can often be diluted 1:1 in TBS without a significant loss of performance. This immunohistochemistry protein blocker consists of proprietary blocking agents and stabilizers prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI). The buffer is pH adjusted to pH 7.6.
Purity/Specifity
This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry