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Supporting Reagents  >  Blocking Solutions and BSA
 

Blocking Buffer (2X) For Fluorescent Western Blotting

Code

MB-070-0050
Size

50 mL
Price

$39.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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This blocking buffer is specifically designed for western blotting using fluorochrome conjugated antibodies and can be used for membrane blocking and to dilute both primary and secondary antibodies.  See www.rockland-inc.com for specific protocols.  This buffer was prepared using ultra-pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerisol added as an antimicrobial agent.
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Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: IRDye Blocking Buffer, Fluorescent Blocking Buffer, Blocking Solution

Blocking Buffer (2X) For Fluorescent Western Blotting Properties

Description
Blocking Buffer (2X) for Fluorescent Western Blotting - MB-070-0050
Application
Western Blot : User Defined
Other Dilution: Optimum performance is achieved using this product undiluted. However, this blocking solution can often be diluted 1:1 in TBS without a significant loss of performance.
Blocker Type
Other
Substrate/Enzyme Type
Blocking Reagent
Product Type
Blocking Reagent
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Label
Buffer
See application note.
Preservative
Thimerosal is added as an antimicrobial agent.
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Lot#  

Blocking Buffer (2X) For Fluorescent Western Blotting Description

Background
Western Blot Blocking Buffer is ideal for infrared Western Blotting. Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background.  IRDye™800, IRDye™700DX, Alexa Fluor® 680 and Cy5.5™ antibody and reagent conjugates are specifically designed for protein detection methods that use longer-wavelength, near-infrared (IR) fluorophores to visualize proteins in western blotting and other applications.  IRDye™800 and IRDye™700DX fluoresce outside the range of most autofluorescence and therefore specific signal is sharply evident from any background giving the best possible signal-to-noise ratio.  Detection levels in the picogram range on Western blots rival the sensitivity of chemiluminescence on film.  IRDye™800 conjugates are also suitable for immunofluorescence microscopy using commercially available excitation/emission filters in the 780nm/820nm range.  Dual simultaneous labeling in western blots or microscopy is achieved when IRDye™800 conjugates are used in conjunction with IRDye™700 or Cy5.5™ conjugates. IRDye™800 and IRDye™700DX conjugates provide an ultra-sensitive and convenient alternative to standard chemiluminescent protein detection methods, as well as a valuable tool for multicolor imaging. Once reacted with the membrane and dried, IRDye™800 and IRDye™700DX conjugated antibody-protein complexes are very stable, and membranes can be stored protected from light, re-washed and/or rescanned.
Storage Condition
Store blocking buffer at 4° C prior to opening. DO NOT FREEZE.
Application Note
This product is a 2X concentrated stock solution.  Prepare a 1X working solution by diluting 1 part 2X concentrate with 1 parts TBS or equivalent. This buffer was meticulously prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerisol added as an antimicrobial agent.
Purity/Specifity
Blocking buffer is specifically formulated to achieve superior reproducible western blotting images using this system.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
Gordon, R; Hogan, CE; Neal, ML; Anantharam, V; Kanthasamy, AG; Kanthasamy , A. A simple magnetic separation method for high-yield isolation of pure primary microglia. Journal of Neuroscience Methods 194 (2011) 287–296
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry